Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents.
Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents.
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Notch信号决定了啮齿动物两种主要类型的犁鼻神经元的细胞命运。
DOI：
10.1242/dev.200448
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发表时间：
2022-07-01
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影响因子：
--
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文献类型：
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陆生脊椎动物寻找食物和交配对象以及躲避捕食者的能力依赖于对化学感觉信息的探测。负责社会和性行为的符号化学物质是由犁鼻器官（VNO）的化学感觉神经元检测到的，它将信息传递给附属嗅球。大多数哺乳动物的犁鼻感觉上皮包含一个统一的犁鼻系统；然而，啮齿动物和有袋动物已经发展出更复杂的双犁鼻系统，其中包含表达V1R或V2R家族受体的犁鼻感觉神经元（VSNs）。在啮齿类动物中，V1R/顶端和V2R/基部VSNs起源于一个共同的祖细胞池。通过单细胞rna测序，我们鉴定了在VSN分化二分法下神经元前体之间Notch1受体和Dll4配体的差异表达。我们的实验表明，Notch信号是V2R/基底VSNs有效分化所必需的。事实上，神经元祖细胞的Notch1功能缺失将它们转向V2R/根尖，而Notch1功能的获得将前体重定向到V2R/基底。我们的研究结果表明，Notch信号在触发鼠类VNO的二元分化二分法中起着关键作用。摘要：单细胞RNA测序揭示了Dll4-Notch1信号在啮齿动物耙鼻器V1R+/顶端和V2R+/基底感觉神经元形成中的重要作用。
The ability of terrestrial vertebrates to find food and mating partners, and to avoid predators, relies on the detection of chemosensory information. Semiochemicals responsible for social and sexual behaviors are detected by chemosensory neurons of the vomeronasal organ (VNO), which transmits information to the accessory olfactory bulb. The vomeronasal sensory epithelium of most mammalian species contains a uniform vomeronasal system; however, rodents and marsupials have developed a more complex binary vomeronasal system, containing vomeronasal sensory neurons (VSNs) expressing receptors of either the V1R or V2R family. In rodents, V1R/apical and V2R/basal VSNs originate from a common pool of progenitors. Using single cell RNA-sequencing, we identified differential expression of Notch1 receptor and Dll4 ligand between the neuronal precursors at the VSN differentiation dichotomy. Our experiments show that Notch signaling is required for effective differentiation of V2R/basal VSNs. In fact, Notch1 loss of function in neuronal progenitors diverts them to the V1R/apical fate, whereas Notch1 gain of function redirects precursors to V2R/basal. Our results indicate that Notch signaling plays a pivotal role in triggering the binary differentiation dichotomy in the VNO of rodents. Summary: Single cell RNA sequencing in the vomeronasal organ reveals a crucial role for Dll4-Notch1 signaling in the formation of V1R+/apical and V2R+/basal vomeronasal sensory neurons of rodents.
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影响因子：
8
作者：
Imayoshi, Itaru;Shimojo, Hiromi;Sakamoto, Masayuki;Ohtsuka, Toshiyuki;Kageyama, Ryoichiro
通讯作者：
Kageyama, Ryoichiro
影响因子：
5.3
作者：
Herrick, Daniel B.;Guo, Zhen;Schwob, James E.
通讯作者：
Schwob, James E.
影响因子：
7.7
作者：
Cheung L;Le Tissier P;Goldsmith SG;Treier M;Lovell-Badge R;Rizzoti K
通讯作者：
Rizzoti K
影响因子：
3.7
作者：
Flanagan KA;Webb W;Stowers L
通讯作者：
Stowers L
DOI：
10.1242/dev.005868
发表时间：
2007-10
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Del Barrio MG;Taveira-Marques R;Muroyama Y;Yuk DI;Li S;Wines-Samuelson M;Shen J;Smith HK;Xiang M;Rowitch D;Richardson WD
通讯作者：
Richardson WD