Analysis of male pheromones that accelerate female reproductive organ development.
Analysis of male pheromones that accelerate female reproductive organ development.
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DOI：
10.1371/journal.pone.0016660
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发表时间：
2011-02-08
期刊：
影响因子：
3.7
通讯作者：
Stowers L
中科院分区：
文献类型：
--
作者：
Flanagan KA;Webb W;Stowers L
雄性气味会影响雌性的生殖生理。在老鼠身上，男性尿液的气味会导致女性青春期提前开始。此前已有报道称，几种挥发性信息素和蛋白质信息素都具有这种生物活性。在这里，我们对近亲交配的BALB/CJ雌性进行生物测试，以研究信息素加速的子宫生长，这是青春期的一个发育标志。我们评估了缺乏特殊感觉转导通道TrpC2的野生型和突变小鼠的反应，发现TrpC2功能对于信息素介导的子宫生长是必要的。我们通过直接生物测定分析了以前确定的加速青春期的信息素的相对有效性，发现没有一种信息素能显着促进BALB/CJ女性的子宫生长。作为对这一分析的补充，我们设计了一种从男性尿液中部分纯化子宫生长生物活性的策略，并将其应用于从三个不同的实验室菌株中纯化生物活性。这三个菌株的活性部分的生化特征都与已知的信息素不一致。当直接分析时，我们无法在尿液中检测到先前已知的信息素，这些信息素会导致子宫生长。我们的分析表明，男性为提早女性青春期而释放的信息素仍有待鉴定。
Male odors can influence a female's reproductive physiology. In the mouse, the odor of male urine results in an early onset of female puberty. Several volatile and protein pheromones have previously been reported to each account for this bioactivity. Here we bioassay inbred BALB/cJ females to study pheromone-accelerated uterine growth, a developmental hallmark of puberty. We evaluate the response of wild-type and mutant mice lacking a specialized sensory transduction channel, TrpC2, and find TrpC2 function to be necessary for pheromone-mediated uterine growth. We analyze the relative effectiveness of pheromones previously identified to accelerate puberty through direct bioassay and find none to significantly accelerate uterine growth in BALB/cJ females. Complementary to this analysis, we have devised a strategy of partial purification of the uterine growth bioactivity from male urine and applied it to purify bioactivity from three different laboratory strains. The biochemical characteristics of the active fraction of all three strains are inconsistent with that of previously known pheromones. When directly analyzed, we are unable to detect previously known pheromones in urine fractions that generate uterine growth. Our analysis indicates that pheromones emitted by males to advance female puberty remain to be identified.
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DOI：
10.1073/pnas.96.10.5791
发表时间：
1999-05-11
影响因子：
11.1
作者：
Liman, ER;Corey, DP;Dulac, C
通讯作者：
Dulac, C
影响因子：
7
作者：
Cutler, Gene;Marshall, Lisa A.;Kassner, Paul D.
通讯作者：
Kassner, Paul D.
影响因子：
64.8
作者：
Chamero, Pablo;Marton, Tobias F.;Stowers, Lisa
通讯作者：
Stowers, Lisa
影响因子：
64.8
作者：
Haga, Sachiko;Hattori, Tatsuya;Touhara, Kazushige
通讯作者：
Touhara, Kazushige
影响因子：
3.3
作者：
GAGNAIRE, F;AZIM, S;DECEAURRIZ, J
通讯作者：
DECEAURRIZ, J