Single‐Cell Glycan and RNA Sequencing (scGR‐seq)
Single‐Cell Glycan and RNA Sequencing (scGR‐seq)
复制标题
单细胞聚糖和 RNA 测序 (scGR-seq)
DOI：
10.1002/cpz1.777
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Tateno Hiroaki
中科院分区：
文献类型：
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作者：
Odaka Haruki;Tateno Hiroaki
单细胞多组学分析已成为现代生物学的重要工具，因为它能够揭示单个细胞内复杂的分子异质性。细胞表面聚糖结构的多样性创造了“细胞特征”，随细胞类型和状态的变化而变化。然而，单细胞聚糖分析仍然是一个挑战。本协议提出了一系列技术和程序，通过基于DNA条形码的凝集素测序（scGR - seq），可以在单细胞水平上同时测量聚糖和RNA。这些技术包括(1)DNA条形码凝集素的制备，(2)单细胞聚糖/RNA测序的一步一步方案，以及(3)整合聚糖和RNA数据的数据分析方法。©2023 Wiley期刊公司基本方案1:DNA条形码凝集素的制备基本方案2：单细胞聚糖/RNA测序
Single‐cell multi‐omics analysis has emerged as a crucial tool in modern biology due to its capacity to uncover the intricate molecular heterogeneity within individual cells. Glycan structural diversity on the cell surface creates a "cell signature" that varies by cell type and state. However, single‐cell glycan analysis remains a challenge. This protocol presents a series of techniques and procedures that enable the simultaneous measurements of glycan and RNA at the single‐cell level via DNA‐barcoded lectin‐based sequencing (scGR‐seq). The techniques include (1) the preparation of DNA‐barcoded lectins, (2) a step‐by‐step protocol for single‐cell glycan/RNA sequencing, and (3) a data analysis approach for the integration of glycan and RNA data. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Preparation of DNA‐barcoded lectinsBasic Protocol 2: Single‐cell glycan/RNA sequencing
影响因子：
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作者：
Odaka H;Ozaki H;Tateno H
通讯作者：
Tateno H