scGR-seq: Integrated analysis of glycan and RNA in single cells.
scGR-seq: Integrated analysis of glycan and RNA in single cells.
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DOI：
10.1016/j.xpro.2022.101179
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Tateno H
中科院分区：
文献类型：
--
作者：
Odaka H;Ozaki H;Tateno H
聚糖是在活细胞表面发现的结构多样的分子。该方案详细描述了使用人诱导多能干细胞（hiPSC）和hiPSC衍生的神经祖细胞（NPC）开发的用于单细胞（scGR-seq）中聚糖和RNA的组合分析的系统。scGR-seq由通过测序的基于DNA条形码凝集素的聚糖分析（scGlycan-seq）和单细胞转录组分析（scRNA-seq）组成。scGR-seq将是描绘跨多细胞系统的聚糖的细胞异质性的必要技术。有关此配置文件的使用和执行的完整详细信息，请参阅。用于单细胞中聚糖和RNA的组合分析的方案（scGR-seq）scGR-seq是基于DNA条形码化凝集素的聚糖谱分析hiPSC和NPC的scGR-seq分析被示出为示例scGR-seq适用于生物体中的任何细胞、组织和器官聚糖是在活细胞表面上发现的结构多样性分子。该方案详细描述了使用人诱导多能干细胞（hiPSC）和hiPSC衍生的神经祖细胞（NPC）开发的用于单细胞（scGR-seq）中聚糖和RNA的组合分析的系统。scGR-seq由通过测序的基于DNA条形码凝集素的聚糖分析（scGlycan-seq）和单细胞转录组分析（scRNA-seq）组成。scGR-seq将是描绘跨多细胞系统的聚糖的细胞异质性的必要技术。
Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to. Protocols for combined analysis of glycan and RNA in single cells (scGR-seq) scGR-seq is a DNA-barcoded lectin-based glycan profiling scGR-seq analysis of hiPSCs and NPCs were shown as an example scGR-seq is adaptable for any cells, tissues, and organs across organisms Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems.
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