Differential volatile signatures from skin, naevi and melanoma: a novel approach to detect a pathological process.
Differential volatile signatures from skin, naevi and melanoma: a novel approach to detect a pathological process.
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DOI：
10.1371/journal.pone.0013813
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发表时间：
2010-11-04
期刊：
影响因子：
3.7
通讯作者：
DeFazio RA
中科院分区：
文献类型：
--
作者：
Abaffy T;Duncan R;Riemer DD;Tietje O;Elgart G;Milikowski C;DeFazio RA
早期发现黑色素瘤对降低死亡率具有重要意义。发现新的黑色素瘤生物标志物将改善早期发现和诊断。在这里，我们提出了一种检测皮肤挥发性化合物的新方法。我们使用顶空固相微萃取（HS-SPME）和气相色谱/质谱（GC/MS）来鉴定黑色素瘤、痣和皮肤样品的挥发性特征。我们假设组织的代谢状态改变了挥发性化合物的特征。比较黑色素瘤和良性痣新鲜活检组织释放挥发物的频率分布和表达水平的差异。我们还分析了冷冻组织的挥发性特征，包括皮肤和黑色素瘤。4-甲基癸烷、十二烷和十一烷三种挥发物在新鲜和冷冻黑色素瘤中均优先表达，表明它们是候选生物标志物。通过模糊逻辑分析对冷冻样品进行评估的12个候选生物标志物区分黑色素瘤和皮肤的灵敏度为89%，特异性为90%。我们的结果证明了黑色素瘤中挥发物的差异表达。我们的挥发性代谢组学方法将有助于更好地了解黑色素瘤，并能够基于代谢改变开发新的诊断和治疗策略。
Early detection of melanoma is of great importance to reduce mortality. Discovering new melanoma biomarkers would improve early detection and diagnosis. Here, we present a novel approach to detect volatile compounds from skin. We used Head Space Solid Phase Micro-Extraction (HS-SPME) and gas chromatography/mass spectrometry (GC/MS) to identify volatile signatures from melanoma, naevi and skin samples. We hypothesized that the metabolic state of tissue alters the profile of volatile compounds. Volatiles released from fresh biopsy tissue of melanoma and benign naevus were compared based on their difference in frequency distribution and their expression level. We also analyzed volatile profiles from frozen tissue, including skin and melanoma. Three volatiles, 4-methyl decane, dodecane and undecane were preferentially expressed in both fresh and frozen melanoma, indicating that they are candidate biomarkers. Twelve candidate biomarkers evaluated by fuzzy logic analysis of frozen samples distinguished melanoma from skin with 89% sensitivity and 90% specificity. Our results demonstrate proof-of-principle that there is differential expression of volatiles in melanoma. Our volatile metabolomic approach will lead to a better understanding of melanoma and can enable development of new diagnostic and treatment strategies based on altered metabolism.
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影响因子：
6
作者：
Horst, Basil;Gruvberger-Saal, Sofia K.;Celebi, Julide Tok
通讯作者：
Celebi, Julide Tok
DOI：
10.1016/j.jchromb.2004.05.015
发表时间：
2004-09-05
影响因子：
3
作者：
Deng, CH;Zhang, XM;Li, N
通讯作者：
Li, N
影响因子：
6.5
作者：
BAMSHAD, J;LERNER, AB;MCGUIRE, JS
通讯作者：
MCGUIRE, JS
影响因子：
15
作者：
Lee, Bobby W. K.;Sun, He G.;Zang, Tianzhu;Kim, Byung Ju;Alfaro, Joshua F.;Zhou, Zhaohui Sunny
通讯作者：
Zhou, Zhaohui Sunny
影响因子：
7.1
作者：
Benevenga, Norlin J.
通讯作者：
Benevenga, Norlin J.