Guanine tetrad and palindromic sequence play critical roles in the RNA dimerization of bovine foamy virus
Guanine tetrad and palindromic sequence play critical roles in the RNA dimerization of bovine foamy virus
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鸟嘌呤四联体和回文序列在牛泡沫病毒RNA二聚化中发挥关键作用
DOI：
10.1007/s00705-007-1047-5
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发表时间：
2007-08
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
逆转录病毒具有二倍体基因组，这是独一无二的。然而，连接这两个RNA分子的RNA序列和结构是不同的。为了确定牛泡沫病毒(BFV)的二聚体连锁位点，利用互补DNA干扰BFV的RNA二聚化。我们发现在一个53个碱基的RNA片段中有两个位点，分别命名为SI和SII，对于BFV的体外二聚是必不可少的。SI由一个潜在的鸟嘌呤四聚体(GGGGC)组成，与引物结合位点重叠，而SII由15个核苷酸组成，包括一个回文序列UCCCUAGGGA。掩蔽这两个位点中的任何一个，都会完全消除RNA二聚体的形成。此外，将SII基因的缺失引入到BFV感染性DNA克隆中，我们发现SII基因的缺失显著增加了BFV反式激活因子Borf-1的表达。有趣的是，我们还发现，这种缺失消除了病毒的传染性。这些结果表明，二聚作用可能在BFV的生活史中发挥着独特的作用。
Retroviruses are unique in having a diploid genome. However, the RNA sequences and structures that link the two RNA molecules are different. To identify the dimer linkage site of bovine foamy virus (BFV), complementary DNAs were used to interfere with RNA dimerization of BFV. We found that two sites, designated SI and SII, within a 53-base RNA fragment, were essential for BFV dimerizationin vitro. SI consists of a potential guanine tetrad (GGGGC), which overlaps the primer binding site, while SII contains 15 nucleotides including a palindromic sequence, UCCCUAGGGA. Masking either of the sites completely abolished RNA dimer formation. Furthermore, a deletion of SII was introduced into a BFV infectious DNA clone; we found that deletion of SII significantly increased expression of BFV transactivator Borf-1. Interestingly, we also found that this deletion abolished viral infectivity. These results suggest that dimerization might play a unique role in the BFV life cycle.
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影响因子：
5.4
作者：
Y. Feng;W. Fu;A. Winter;J. G. Levin;A. Rein
通讯作者：
Y. Feng;W. Fu;A. Winter;J. G. Levin;A. Rein
影响因子：
5.4
作者：
Tobaly-Tapiero, J;Bittoun, P;Saïb, A
通讯作者：
Saïb, A
影响因子：
5.4
作者：
Murray, SM;Picker, LJ;Linial, ML
通讯作者：
Linial, ML
DOI：
--
发表时间：
2000
期刊：
Chinese journal of virology
影响因子：
--
作者：
Liu Jiajian;Wang Shizhen;Zhangcheng Li;Qiao Wentao;Chen Qi-min;Geng Yunqi
通讯作者：
Liu Jiajian;Wang Shizhen;Zhangcheng Li;Qiao Wentao;Chen Qi-min;Geng Yunqi
影响因子：
5.7
作者：
Ennifar, E;Yusupov, M;Dumas, P
通讯作者：
Dumas, P