Investigation of 6-[¹⁸F]-fluoromaltose as a novel PET tracer for imaging bacterial infection.
Investigation of 6-[¹⁸F]-fluoromaltose as a novel PET tracer for imaging bacterial infection.
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DOI：
10.1371/journal.pone.0107951
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gambhir SS
中科院分区：
文献类型：
--
作者：
Gowrishankar G;Namavari M;Jouannot EB;Hoehne A;Reeves R;Hardy J;Gambhir SS
尽管核医学领域取得了进展，但细菌感染的成像仍然是一个挑战。现有试剂的灵敏度和特异性较差。在这项研究中，我们研究了一种新的PET（正电子发射断层扫描）示踪剂，克服了这些限制的潜力。合成了6-[18F]-氟麦芽糖。在细菌和哺乳动物培养物中评价了其体外行为。从小鼠模型中获得示踪剂的详细药代动力学和生物分布曲线。6-[18F]-氟麦芽糖被多种致病菌株吸收。它不被哺乳动物癌细胞系吸收。在细菌性肌炎的鼠模型中，6-[18F]-氟麦芽糖保留在感染的肌肉中。它不会积聚在发炎的组织中。我们已经表明，6-[18F]-氟麦芽糖可用于成像细菌感染在体内具有高特异性。我们相信这类药物将对患者的临床管理产生重大影响。
Despite advances in the field of nuclear medicine, the imaging of bacterial infections has remained a challenge. The existing reagents suffer from poor sensitivity and specificity. In this study we investigate the potential of a novel PET (positron emission tomography) tracer that overcomes these limitations. 6-[18F]-fluoromaltose was synthesized. Its behavior in vitro was evaluated in bacterial and mammalian cultures. Detailed pharmacokinetic and biodistribution profiles for the tracer were obtained from a murine model. 6-[18F]-fluoromaltose is taken up by multiple strains of pathogenic bacteria. It is not taken up by mammalian cancer cell lines. 6-[18F]-fluoromaltose is retained in infected muscles in a murine model of bacterial myositis. It does not accumulate in inflamed tissue. We have shown that 6-[18F]-fluoromaltose can be used to image bacterial infection in vivo with high specificity. We believe that this class of agents will have a significant impact on the clinical management of patients.
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DOI：
10.1111/j.1432-1033.1980.tb04758.x
发表时间：
1980-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FERENCI, T
通讯作者：
FERENCI, T
影响因子：
3.1
作者：
Jones, Shari A.;Jorgensen, Mathias;Conway, Tyrrell
通讯作者：
Conway, Tyrrell
影响因子：
4.7
作者：
Sachin, Kalme;Kim, Eun-Mi;Kim, Dong Wook
通讯作者：
Kim, Dong Wook
影响因子：
56.9
作者：
Hardy, J;Francis, KP;Contag, CH
通讯作者：
Contag, CH
影响因子：
41.2
作者：
Ning X;Lee S;Wang Z;Kim D;Stubblefield B;Gilbert E;Murthy N
通讯作者：
Murthy N