Studies on NADH(NADPH)-cytochrome c reductase (FMN-containing) from yeast: steady-state kinetic properties of the flavoenzyme from top-fermenting ale yeast.
Studies on NADH(NADPH)-cytochrome c reductase (FMN-containing) from yeast: steady-state kinetic properties of the flavoenzyme from top-fermenting ale yeast.
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酵母 NADH(NADPH)-细胞色素 c 还原酶（含 FMN）的研究：顶部发酵艾尔酵母黄素酶的稳态动力学特性。
DOI：
10.1016/0003-9861(86)90214-6
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发表时间：
1986
影响因子：
3.9
通讯作者：
Kuby,SA
中科院分区：
文献类型：
--
作者：
Johnson,MS;Kuby,SA
酵母中NADH(NADPH)-细胞色素还原酶（FMN-containing）的稳态动力学研究[M. S. Johnson and S. A. Kuby(1985)]。医学杂志。Chem.260, 12341-12350)导致了一个假设的三底物随机有序杂交机制，其中NAD(P)H和FMN随机添加，并且很可能以稳态方式添加，随后是细胞色素的有序添加。从这一机理推导出了动力学参数。阿伦尼乌斯图显示NADH和NADPH作为底物还原剂之间存在较大差异。甲萘醌加速了细胞色素的还原和o2的摄取，但维生素k1和辅酶q10作为电子介质是无效的，可能是由于它们的不溶性。以NADPH为底物还原剂时，电子受体的还原速率顺序为铁氰化物> DCIP >细胞色素>氧；甲萘醌的特异性序列为细胞色素>铁氰化> DCIP >氧。添加NADH后，其顺序为铁氰化>细胞色素>氧> DCIP，添加甲萘醌后为细胞色素>铁氰化>氧> DCIP。细胞色素b5在缺氧条件下也减少。没有观察到转氢酶活性，但NADH和NADPH的还原硫代烟酰胺类似物作为底物。超氧化物歧化酶能抑制空气中50%的细胞色素还原，但O2⪰2不是细胞色素还原所必需的，这可以从缺氧时细胞色素还原速率的增加中得到证明。与氧反应的产物是H2O2。
A study of the steady-state kinetics of NADH(NADPH)-cytochromecreductase (FMN-containing) from ale yeast (M. S. Johnson and S. A. Kuby (1985)J. Biol. Chem.260, 12341–12350) has led to a postulated three-substrate random-ordered hybrid mechanism, where NAD(P)H and FMN add randomly and very likely in a steady-state fashion, followed by an ordered addition of cytochromec. Kinetic parameters have been derived from this mechanism. Arrhenius plots showed large differences between NADH and NADPH, as the substrate-reductant. Menadione accelerated cytochromecreduction and also O2uptake, but vitamin K1and coenzyme Q10were ineffective as electron mediators, possibly as a result of their insolubility. With NADPH as the substrate-reductant, the order of the rate of reduction of electron acceptors was ferricyanide > DCIP > cytochromec> oxygen; with menadione, the specificity sequence was cytochromec> ferricyanide > DCIP > oxygen. With NADH, the order was ferricyanide > cytochromec> oxygen > DCIP, which changed to cytochromec> ferricyanide > oxygen > DCIP on addition of menadione. Cytochromeb5was also reduced in the absence of oxygen. No transhydrogenase activity was observed, but the reduced thionicotinamide analogs of NADH and NADPH acted as substrates. Superoxide dismutase inhibited cytochromecreduction in air by 50%, but O⪰2was not necessary for cytochromecreduction, as evidenced by the increase in rate in the absence of O2. The product of the reaction with oxygen appeared to be H2O2.
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DOI：
10.1159/000469611
发表时间：
1983
期刊：
Enzyme
影响因子：
--
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
R. Roy
酵母中 NADH (NADPH)-细胞色素 c 还原酶（含 FMN）的研究。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Johnson,MS;Kuby,SA
通讯作者：
Kuby,SA
还原型二磷酸吡啶核苷酸的氧化。
DOI：
10.1016/s0021-9258(19)77327-x
发表时间：
1958
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Schellenberg;L. Hellerman
通讯作者：
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一种对二磷酸吡啶核苷酸具有特异性的微粒体细胞色素还原酶。
DOI：
10.1016/s0021-9258(18)65247-0
发表时间：
1956
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
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通讯作者：
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