Molecular and immunologic analysis of a chronic lymphocytic leukemia case with antibodies against human T‐cell leukemia virus
Molecular and immunologic analysis of a chronic lymphocytic leukemia case with antibodies against human T‐cell leukemia virus
复制标题
一例慢性淋巴细胞白血病患者 T 细胞白血病病毒抗体的分子和免疫学分析
作者：
J. Clark;B. Hahn;D. Mann;F. Wong;M. Popovič;E. Richardson;D. Strong;W. Lofters;W. Blattner;W. Gibbs;R. Gallo
人类T细胞白血病病毒I型（HTLV - I）是一种独特的、外源性的、水平传播的逆转录病毒，它是向T细胞转移的，并且与一种特定类型的起源于成熟T细胞的侵袭性白血病/淋巴瘤有关。在牙买加的一项淋巴细胞恶性肿瘤调查中，17例慢性淋巴细胞白血病（CLL）患者中有6例也发现HTLV‐1抗体，这提高了病因学关系的可能性。对其中一名患者进行了进一步的研究，以澄清疾病的性质和可能的病毒关系。外周血细胞表面标记分析表明，循环淋巴细胞主要为B细胞。DNA克隆探针对这些外周血恶性B细胞的HTLV - I前病毒完整基因组进行分析，结果显示整合病毒阴性。用外周血培养T细胞系。通过检测表达的病毒特异性gag蛋白p - 19和前病毒DNA，证实HTLV - I在培养的T细胞系中存在。因此，HTLV - 1感染的T细胞存在时可发生B淋巴细胞恶性肿瘤，提示可能存在间接的白血病发生机制。
The human T‐cell leukemia virus type‐I (HTLV‐I) is a unique, exogenous, horizontally transmitted retrovirus which is T‐cell tropic, and has been associated with a specific type of aggressive leukemia/lymphoma of mature T‐cell origin. In a survey of lymphoid malignancies in Jamaica, antibodies to HTLV‐I were also found in 6 of 17 patients with chronic lymphocytic leukemia (CLL), raising the possibility of an etiologic relationship. Further studies were undertaken on one of these patients to clarify the nature of the disease and possible virus relationship. Cell surface marker analysis of her peripheral blood cells documented that the majority of circulating lymphocytes were B‐cells. DNA‐cloned probe analysis with a complete HTLV‐I proviral genome of these peripheral malignant B‐cells, was negative for integrated virus. A T‐cell line was established in culture from her peripheral blood. The presence of HTLV‐I in the cultured T‐cell line was established by the detection of expressed viral specific gag protein p‐19 and proviral DNA. Thus, a B‐cell lymphoid malignancy can occur in the presence of HTLV‐I infected T‐cells, suggesting the possibility of an indirect leukemogenic mechanism.
抑制自然逆转录病毒感染中的体液抗体反应。
DOI：
10.1126/science.6302837
发表时间：
1983
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Trainin,Z;Wernicke,D;Ungar-Waron,H;Essex,M
通讯作者：
Essex,M