Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin.
Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin.
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双壁多壁聚（D，L-丙二醇 - 糖糖苷）（PLGA）和聚（L-甲状腺素）（PLLA）纳米颗粒，用于持续释放阿霉素。
DOI：
10.3390/polym13193230
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发表时间：
2021-09-23
期刊：
影响因子：
5
通讯作者：
Bicho A
中科院分区：
文献类型：
--
作者：
Cardoso MM;Peca IN;Lopes T;Gardner R;Bicho A
采用聚(D,L-丙交酯-乙交酯) (PLGA) 和聚(L-丙交酯) (PLLA) 通过溶剂蒸发技术生产含有阿霉素作为模型药物的双壁纳米颗粒 (DWNP)。还生产了含有阿霉素的双壁微粒，使得使用光学和荧光显微镜检查内部形态和药物分布成为可能。产生的微粒呈现出双壁结构，阿霉素溶解在富含 PLGA 的相中。生产的 DWNP 具有非常低的初始爆发值和持续至少 90 天的 DOX 释放，释放率随着 PLLA 量的增加而降低。第 15 天后获得零级释放动力学。结果支持 PLLA 层充当速率控制屏障，并且可以通过增加未负载的外层厚度来延迟阿霉素从载药内部 PLGA 核心的扩散。所产生的未负载的双壁纳米粒子用于CHO细胞的体外测试，并证明它们是无毒的，而负载阿霉素的双壁纳米粒子引起了很大的细胞活力，并且在体外测试时降低了细胞活力。
Double-walled nanoparticles (DWNPs), containing doxorubicin as a model drug, were produced using poly-(D,L-lactide-co-glycolide) (PLGA) and poly(L-lactide) (PLLA) by the solvent evaporation technique. Double-walled microparticles containing doxorubicin were also produced to make possible the examination of the inner morphology and drug distribution using optical and fluorescence microscopy. The produced microparticles present a double-walled structure with doxorubicin solubilized in the PLGA-rich phase. The DWNPs produced present very low initial burst values and a sustained DOX release for at least 90 days with release rates decreasing with the increase in the PLLA amount. Zero-order release kinetics were obtained after day 15. The results support that the PLLA layer acts as a rate control barrier and that the diffusion of doxorubicin from the drug-loaded inner PLGA core can be retarded by an increase in the thickness of the unloaded outer layer. The unloaded double-walled nanoparticles produced were used in in vitro tests with CHO cells and demonstrate that they are nontoxic, while the double-walled nanoparticles loaded with doxorubicin caused a great cellular viability and decreased when tested in vitro.
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