Immunohistochemical Evidence that Angiotensins I and II Are Formed by Intracellular Mechanism in Juxtaglomerular Cells
Immunohistochemical Evidence that Angiotensins I and II Are Formed by Intracellular Mechanism in Juxtaglomerular Cells
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免疫组织化学证据表明血管紧张素 I 和 II 是通过肾小球旁细胞的细胞内机制形成的
DOI：
10.1161/01.hyp.4.3_pt_2.70
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发表时间：
1982
期刊：
影响因子：
8.3
通讯作者：
Y. Takii
中科院分区：
文献类型：
--
作者：
M. Celio;R. Workman;Y. Takii
血管紧张素II（ALL）免疫反应性的存在，在大鼠肾肾小球（JG）细胞，这已被证明以前的免疫组织化学研究，可以解释为细胞内合成的产品或由受体结合的所有起源于血浆的内化。为了解决这两个替代机制，尝试了识别AI在大鼠肾脏JG细胞免疫组化染色使用特定的抗体AI。在正常大鼠肾组织中未检测到Al样免疫反应，而在血管紧张素转换酶抑制剂MK-421或Captopril处理的大鼠肾组织中，JG细胞显示Al样免疫反应。通过相邻连续切片上使用的相应抗原的特异性抗体，在相同细胞中证明了肾素和All样免疫反应性的存在。这些发现支持了AH形成的细胞内机制，并表明细胞内的肾素血管紧张素系统，可能是从细胞外系统分离。（高血压4（增刊II）：II-70-II-74，1982年）
The existence of angiotensin II (All) immunoreactivity in juxtaglomerular (JG) cells of rat kidney, which has been demonstrated previously by immunohistochemical studies, can be explained either as the product of intracellular synthesis or by the internalization of receptor-bound All originating in plasma. To resolve these two alternative mechanisms, attempts were made to identify AI in JG cells of rat kidney by immunohistochemical staining using specific antibodies to AI. Although Al-like immunoreactivity was not detected in normal rat kidney, rats treated with the angiotensin-conrerting enzyme inhibitors, MK-421 or captopril, showed Al-like Immunoreactivity in JG cells. The presence of renin and All-like immunoreactivity was demonstrated in the same cells by specific antibodies to respective antigens used on adjacent serial sections. These findings support an intracellular mechanism of the formation of AH and suggest an intracellular renin angiotensin system, presumably separate from the extracellular system. (Hypertension 4 (suppl II): II-70-II-74, 1982)
血管紧张素 II 的主要抗利尿作用的证明：肾内转化酶抑制对清醒狗的影响。
影响因子：
4.8
作者：
Levens,NR;Peach,MJ;VaughanJr,ED;Carey,RM
通讯作者：
Carey,RM
血管紧张素 II 免疫反应性与肾素共存于肾脏的肾小球旁颗粒细胞中。
DOI：
10.1073/pnas.78.6.3897
发表时间：
1981
影响因子：
11.1
作者：
Celio,MR;Inagami,T
通讯作者：
Inagami,T