Identification and profiling of microRNAs in the embryonic breast muscle of pekin duck.
Identification and profiling of microRNAs in the embryonic breast muscle of pekin duck.
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DOI：
10.1371/journal.pone.0086150
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hou S
中科院分区：
文献类型：
--
作者：
Gu L;Xu T;Huang W;Xie M;Sun S;Hou S
MicroRNA (miRNA) 通过完全或部分结合互补序列来调节基因表达，并在骨骼肌发育中发挥重要作用。然而，miRNAs在鸭胚胎胸肌中的作用尚不清楚。在本研究中，我们通过高通量测序分析了北京鸭胚胎胸肌 E13（孵化第 13 天）、E19 和 E27 的 miRNA 谱。共获得382个miRNA，其中包括359个珍贵鉴定的miRNA和23个新的候选miRNA。对已鉴定的miRNA进行核苷酸偏倚分析表明，北京鸭中的miRNA具有高度保守性。所鉴定的 miRNA 的表达在 E13 和 E19 之间以及 E27 和 E19 之间存在显着差异。使用茎环 qRT-PCR 验证的 15 个已鉴定的 miRNA 可分为三组：在 E19 表达峰值的、在 E19 表达最小的、以及从 E11 到 E27 持续增加的。考虑到E19是胚胎北京鸭胸肌生长最快的阶段，这三组miRNA可能是胸肌生长的潜在促进者、潜在抑制剂和潜在维持者。在23个新颖的miRNA中，新颖的miRNA-8和新颖的miRNA-14在某些阶段表达量最大。对两个新颖的miRNA及其两个靶标（MAP2K1和PPARα）的茎环qRT-PCR分析表明，新颖的mir-8和PPARα的表达在E19时达到最低点，而新颖的mir-14和MAP2K1的表达在E19时达到峰值，表明新颖的miRNA-8和新颖的miRNA-14可能是鸭胚胎胸肌发育的潜在抑制剂和潜在的促进剂。总之，这些结果不仅为鸭胚胎胸肌中miRNA的分布提供了全面的了解，而且为进一步研究miRNA在鸭骨骼肌发育中的作用奠定了基础。
MicroRNAs (miRNAs) regulate gene expression by fully or partially binding to complementary sequences and play important roles in skeletal muscle development. However, the roles of miRNAs in embryonic breast muscle of duck are unclear. In this study, we analyzed the miRNAs profiling in embryonic breast muscle of Pekin duck at E13 (the 13th day of hatching), E19, and E27 by high-throughput sequencing. A total of 382 miRNAs including 359 preciously identified miRNAs 23 novel miRNA candidates were obtained. The nucleotide bias analysis of identified miRNAs showed that the miRNAs in Pekin duck was high conserved. The expression of identified miRNAs were significantly different between E13 and E19 as well as between E27 and E19. Fifteen identified miRNAs validated using stem-loop qRT-PCR can be divided into three groups: those with peak expression at E19, those with minimal expression at E19, and those with continuous increase from E11 to E27. Considering that E19 is the fastest growth stage of embryonic Pekin duck breast muscle, these three groups of miRNAs might be the potential promoters, the potential inhibitors, and the potential sustainer for breast muscle growth. Among the 23 novel miRNAs, novel-miRNA-8 and novel-miRNA-14 had maximal expression at some stages. The stem-loop qRT-PCR analysis of the two novel miRNAs and their two targets (MAP2K1 and PPARα) showed that the expression of novel-mir-8 and PPARα reached the lowest points at E19, while that of novel-mir-14 and MAP2K1 peaked at E19, suggesting novel-miRNA-8 and novel-miRNA-14 may be a potential inhibitor and a potential promoter for embryonic breast muscle development of duck. In summary, these results not only provided an overall insight into the miRNAs landscape in embryonic breast muscle of duck, but also a basis for the further investigation of the miRNAs roles in duck skeletal muscle development.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
3.7
作者：
Cardinali B;Castellani L;Fasanaro P;Basso A;Alemà S;Martelli F;Falcone G
通讯作者：
Falcone G
影响因子：
2.7
作者：
Biggar, Kyle K.;Dubuc, Adrian;Storey, Kenneth
通讯作者：
Storey, Kenneth
影响因子：
9.2
作者：
Caygill, Elizabeth E.;Johnston, Laura A.
通讯作者：
Johnston, Laura A.
影响因子：
3.1
作者：
Callis, Thomas E.;Chen, Jian-Fu;Wan, Da-Zhi
通讯作者：
Wan, Da-Zhi