Biofunctionalized magnetic-vortex microdiscs for targeted cancer-cell destruction.
Biofunctionalized magnetic-vortex microdiscs for targeted cancer-cell destruction.
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作者：
纳米磁性材料为探测细胞力学和激活机械敏感离子通道以及推进癌症治疗提供了令人兴奋的途径。到目前为止，大多数实验工作都使用超顺磁性材料。这份报告描述了第一种方法的基础上接口细胞与光刻定义的microdiscs，拥有一个自旋涡旋基态。当施加交变磁场时，微盘涡旋移动，产生振荡，将机械力传递给细胞。由于癌细胞对凋亡的敏感性降低导致不适当的细胞存活和恶性进展，因此选择性诱导凋亡对于抗癌治疗策略具有重要意义。我们发现，自旋涡旋介导的刺激产生两个戏剧性的效果：受损的细胞膜的完整性，并启动程序性细胞死亡。一个几十赫兹的低频场只施加10分钟就足以在体外摧毁90%的癌细胞。
Nanomagnetic materials offer exciting avenues for probing cell mechanics and activating mechanosensitive ion channels, as well as for advancing cancer therapies. Most experimental works so far have used superparamagnetic materials. This report describes a first approach based on interfacing cells with lithographically defined microdiscs that possess a spin-vortex ground state. When an alternating magnetic field is applied the microdisc vortices shift, creating an oscillation, which transmits a mechanical force to the cell. Because reduced sensitivity of cancer cells toward apoptosis leads to inappropriate cell survival and malignant progression, selective induction of apoptosis is of great importance for the anticancer therapeutic strategies. We show that the spin-vortex-mediated stimulus creates two dramatic effects: compromised integrity of the cellular membrane, and initiation of programmed cell death. A low-frequency field of a few tens of hertz applied for only ten minutes was sufficient to achieve ~90% cancer-cell destruction in vitro.
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DOI：
10.1084/jem.194.12.1743
发表时间：
2001-12-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Kawakami K;Kawakami M;Snoy PJ;Husain SR;Puri RK
通讯作者：
Puri RK
影响因子：
8.6
作者：
Cowburn, RP;Koltsov, DK;Tricker, DM
通讯作者：
Tricker, DM
影响因子：
3.2
作者：
Mpoke, SS;Wolfe, J
通讯作者：
Wolfe, J
影响因子：
2.2
作者：
Afrin, R;Yamada, T;Ikai, A
通讯作者：
Ikai, A
影响因子：
21.3
作者：
Boehning, D;Patterson, RL;Snyder, SH
通讯作者：
Snyder, SH