Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli.
Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli.
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DOI：
10.1093/nar/gkm456
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发表时间：
2007
影响因子：
14.9
通讯作者：
Burgess RR
中科院分区：
文献类型：
--
作者：
Zhao K;Liu M;Burgess RR
细菌鞭毛的运动性和趋化性帮助细胞到达最有利的环境，并在响应外部刺激时成功地与其他微生物竞争。大肠杆菌是一种活动的革兰氏阴性菌，其鞭毛调控由主调控因子FlhDC和第二调控因子——鞭毛特异性sigma因子σF共同调控。为了明确这两种调节因子的生理作用，我们开展了转录谱分析实验，在全基因组基础上鉴定这两种调节因子控制的基因。此外，随着碳源质量的降低，CRP活性增加导致FlhDC表达增加的同步模式，以及在质量较差的碳源中，运动性活性与运动性和趋化性基因的激活明显耦合，突出了CRP激活在允许大肠杆菌逐渐将其有限储备投入更多以寻找更好条件方面的重要性。在适应各种碳源时，活动细菌通过增加鞭毛活动来进行战术反应，但限制昂贵的鞭毛合成，这表明它有能力在营养贫乏的环境中战略性地利用宝贵的能量来最大化生存。
Bacterial flagellar motility and chemotaxis help cells to reach the most favorable environments and to successfully compete with other micro-organisms in response to external stimuli. Escherichia coli is a motile gram-negative bacterium, and the flagellar regulon in E. coli is controlled by a master regulator FlhDC as well as a second regulator, flagellum-specific sigma factor, σF. To define the physiological role of these two regulators, we carried out transcription profiling experiments to identify, on a genome-wide basis, genes under the control of these two regulators. In addition, the synchronized pattern of increasing CRP activity causing increasing FlhDC expression with decreasing carbon source quality, together with the apparent coupling of motility activity with the activation of motility and chemotaxis genes in poor quality carbon sources, highlights the importance of CRP activation in allowing E. coli to devote progressively more of its limited reserves to search out better conditions. In adaptation to a variety of carbon sources, the motile bacteria carry out tactical responses by increasing flagellar operation but restricting costly flagellar synthesis, indicating its capability of strategically using the precious energy in nutrient-poor environments for maximizing survival.
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影响因子：
64.8
作者：
BURGESS, RR;TRAVERS, AA;BAUTZ, EKF
通讯作者：
BAUTZ, EKF
影响因子：
5.6
作者：
Claret, L;Hughes, C
通讯作者：
Hughes, C
影响因子：
2.7
作者：
HASTJARJO, T;SILBERBERG, A;HURSH, SR
通讯作者：
HURSH, SR
影响因子：
64.5
作者：
Karlinsey, JE;Lonner, J;Hughes, KT
通讯作者：
Hughes, KT
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL