Induction of a torpor-like hypothermic and hypometabolic state in rodents by ultrasound.
Induction of a torpor-like hypothermic and hypometabolic state in rodents by ultrasound.
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DOI：
10.1038/s42255-023-00804-z
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发表时间：
2023-05
影响因子：
20.8
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Yang, Yaoheng;Yuan, Jinyun;Field, Rachael L.;Ye, Dezhuang;Hu, Zhongtao;Xu, Kevin;Xu, Lu;Gong, Yan;Yue, Yimei;Kravitz, Alexxai V.;Bruchas, Michael R.;Cui, Jianmin;Brestoff, Jonathan R.;Chen, Hong
休眠是一种能量保存状态，动物在这种状态下会显著降低代谢率和体温，以在恶劣的环境条件下生存。在这里，我们报告了无创的，精确的和安全的诱导torpor-like低温和低代谢状态的啮齿动物远程经颅超声刺激下丘脑视前区（POA）。我们通过对超声刺激的闭环反馈控制和自动体温检测，在小鼠中实现了持久（>24小时）的类休眠状态。超声诱导的低温和低代谢（UIH）是由POA神经元激活触发的，涉及背内侧下丘脑作为下游脑区，随后抑制产热棕色脂肪组织。POA神经元的单核RNA测序揭示TRPM 2作为超声敏感离子通道，其敲低抑制UIH。我们还表明，UIH是可行的，在一个非迟钝的动物，大鼠。我们的研究结果建立UIH作为一个有前途的技术，无创和安全的诱导torpor-like状态。使用超声波激活下丘脑中的非侵入性特异性神经元，在啮齿动物中诱导暂时的低温和低代谢状态。
Torpor is an energy-conserving state in which animals dramatically decrease their metabolic rate and body temperature to survive harsh environmental conditions. Here, we report the noninvasive, precise and safe induction of a torpor-like hypothermic and hypometabolic state in rodents by remote transcranial ultrasound stimulation at the hypothalamus preoptic area (POA). We achieve a long-lasting (>24 h) torpor-like state in mice via closed-loop feedback control of ultrasound stimulation with automated detection of body temperature. Ultrasound-induced hypothermia and hypometabolism (UIH) is triggered by activation of POA neurons, involves the dorsomedial hypothalamus as a downstream brain region and subsequent inhibition of thermogenic brown adipose tissue. Single-nucleus RNA-sequencing of POA neurons reveals TRPM2 as an ultrasound-sensitive ion channel, the knockdown of which suppresses UIH. We also demonstrate that UIH is feasible in a non-torpid animal, the rat. Our findings establish UIH as a promising technology for the noninvasive and safe induction of a torpor-like state. Using ultrasound to activate noninvasively specific neurons in the hypothalamus, a temporary hypothermic and hypometabolic state is induced in rodents.
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影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
64.5
作者：
Fedorenko A;Lishko PV;Kirichok Y
通讯作者：
Kirichok Y
DOI：
10.1523/jneurosci.1240-11.2011
发表时间：
2011-07-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Jinka TR;Tøien Ø;Drew KL
通讯作者：
Drew KL
影响因子：
1.8
作者：
Cerri, Matteo
通讯作者：
Cerri, Matteo
影响因子：
56.9
作者：
Blackstone, E;Morrison, M;Roth, MB
通讯作者：
Roth, MB