Effects of highly selective sensory/motor nerve injury on bone metabolism and bone remodeling in rats
Effects of highly selective sensory/motor nerve injury on bone metabolism and bone remodeling in rats
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高选择性感觉/运动神经损伤对大鼠骨代谢和骨重塑的影响
DOI：
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发表时间：
2022-12
影响因子：
1.9
通讯作者：
Bo Yu
中科院分区：
文献类型：
--
作者：
Yadong Yan;Juan Zhou;Chen Liang;Qi Xiao;Yan Chen;Bo Yu
目的：探讨选择性感觉/运动神经损伤影响骨代谢和骨重建的机制。方法：分别对大鼠右侧L（4-6）感觉/运动神经行后根切断术（PRG）、前根切断术（ARG）或前后根联合切断术（APRG），建立选择性感觉/运动神经损伤大鼠模型。假手术（SOG）大鼠作为对照。术后8周，收集坐骨神经、脊髓节段L（5）和胫骨组织进行分析。研究结果：ARG组骨小梁完整性受损，骨小梁数量减少，破骨细胞数量增多。ARG激活NF-κ B和PPAR-gamma通路，抑制Wnt/beta-catenin通路。ARG组骨代谢高转换。PRG组骨小梁形态变细，破骨细胞数量增多。PRG组NF-κ B通路激活，OPG/RANKL比值降低。PRG组破骨细胞活化，成骨细胞活性降低，骨代谢转换降低。此外，APRG组的骨代谢与ARG组相似。结论：后根和前根切断导致大鼠不同程度的骨质疏松，其机制可能与调节Wnt/beta-catenin、NF-κ B和PPAR-gamma信号通路有关。
OBJECTIVES: This work aimed to investigate the mechanism of selective sensory/motor nerve injury in affecting bone metabolism and remodeling. METHODS: The selective sensory/motor nerve injury rat model was constructed through posterior rhizotomy (PRG), anterior rhizotomy (ARG), or anterior combined with posterior rhizotomy (APRG) at the L(4-6) sensory/motor nerves on the right side of rats. Sham-operated (SOG) rats served as control. At 8 weeks after surgery, the sciatic nerves, spinal cord segments L(5) and tibial tissues were collected for analysis. RESULTS: the integrity of trabecular bone was damaged, the number of trabecular bone was decreased and the number of osteoclasts were increased in ARG group. ARG activated NF-kappabeta and PPAR-gamma pathways, and inhibited Wnt/beta-catenin pathway. ARG group exhibited high turnover bone metabolism. In PRG group, the trabecular bone morphology became thinner, and the number of osteoclasts was increased. NF-kappabeta pathway was activated and OPG/RANKL ratio was decreased in PRG group. The activated osteoclasts, reduced osteoblasts activity and lower turnover bone metabolism were observed in PRG group. Additionally, the bone metabolism in APRG group was similar to ARG group. CONCLUSION: The posterior rhizotomy and anterior rhizotomy induced the different degree of osteoporosis in rats, which may attribute to regulate Wnt/beta-catenin, NF-kappabeta and PPAR-gamma signalling pathways.
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影响因子：
5.9
作者：
Go GW
通讯作者：
Go GW
影响因子：
1.9
作者：
K. Lips;V. Kauschke;S. Hartmann;U. Thormann;S. Ray;M. Schumacher;M. Gelinsky;S. Heinemann;T. Hanke;A. Kautz;M. Schnabelrauch;G. Szalay;C. Heiss;R. Schnettler;V. Alt;O. Kilian
通讯作者：
K. Lips;V. Kauschke;S. Hartmann;U. Thormann;S. Ray;M. Schumacher;M. Gelinsky;S. Heinemann;T. Hanke;A. Kautz;M. Schnabelrauch;G. Szalay;C. Heiss;R. Schnettler;V. Alt;O. Kilian
影响因子：
2.2
作者：
Jin A Choi;Hyun-Jin Jin-Hyun-Jin-Jin-2256478452;Samhyun Jung;Eunkyung Yang;Jun-Sub Choi;So-Hyang Chung;Choun-Ki Joo
通讯作者：
Jin A Choi;Hyun-Jin Jin-Hyun-Jin-Jin-2256478452;Samhyun Jung;Eunkyung Yang;Jun-Sub Choi;So-Hyang Chung;Choun-Ki Joo
影响因子：
5.6
作者：
M. Vishal;S. Vimalraj;R. Ajeetha;M. Gokulnath;R. Keerthana;Z. He;N. Partridge;N. Selvamurugan
通讯作者：
M. Vishal;S. Vimalraj;R. Ajeetha;M. Gokulnath;R. Keerthana;Z. He;N. Partridge;N. Selvamurugan
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影响因子：
7.5
作者：
Ge, Xingyun;Li, Zehan;Yu, Jinhua
通讯作者：
Yu, Jinhua