Distribution of virulence genes related to adhesins and toxins in shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan.
Distribution of virulence genes related to adhesins and toxins in shiga toxin-producing Escherichia coli strains isolated from healthy cattle and diarrheal patients in Japan.
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从日本健康牛和腹泻患者中分离出的产志贺毒素大肠杆菌菌株中与粘附素和毒素相关的毒力基因的分布。
DOI：
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发表时间：
2010
影响因子：
1.2
通讯作者：
S. Yamasaki
中科院分区：
文献类型：
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作者：
Yuluo Wu;A. Hinenoya;T. Taguchi;A. Nagita;K. Shima;T. Tsukamoto;Norihiko Sugimoto;M. Asakura;S. Yamasaki
研究了日本产志贺毒素大肠杆菌（STEC）的毒力基因分布。采用集落杂交法检测了232株产志贺毒素大肠杆菌菌株（其中171株来自牛，61株来自人）中与大肠杆菌粘附相关的基因，如eae（大肠杆菌粘附素）、saa（产志贺毒素大肠杆菌自凝集粘连素）、iha（产志贺毒素大肠杆菌同系物粘连素）、efa1（大肠杆菌粘附因子）、lpfA(O113)（长极菌毛）和ehaA（肠出血性大肠杆菌自转运蛋白）。同样，也检查了产毒性cdt（细胞致死膨胀毒素）和subAB（枯草酶细胞毒素）基因的存在。分离株中，lpfA(O113)（99%）、ehaA（95%）、iha（94%）、saa（91%）、subAB（65%）和cdt-V（49%）分别阳性170株、163株、161株、155株、112株和84株，eae（1.2%）和efa1（1.2%）各阳性2株。在人类分离株中，60、59、58和58分别对ehaA（98%）、iha（97%）、efa1（95%）和eae（95%）呈阳性，11、2、2和1分别对lpfA(113)（18%）、saa（3.3%）、cdt-V（3.3%）和subAB（1.6%）呈阳性。因此，在人产STEC分离株efa1和eae中，而在牛产STEC分离株saa中，lpfA（O113）、cdt-V和subAB普遍存在。这些数据表明，某些致病基因在日本源自人类和牛的产志贺毒素大肠杆菌菌株中存在差异。
Shiga toxin-producing Escherichia coli (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., eae (intimin, E. coli attaching and effacing), saa (STEC autoagglutinating adhesin), iha (irgA homologue adhesin), efa1 (E. coli factor for adherence), lpfA(O113) (long polar fimbriae), and ehaA (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic cdt (cytolethal distending toxin), and subAB (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for lpfA(O113) (99%), ehaA (95%), iha (94%), saa (91%), subAB (65%), and cdt-V (49%), respectively, while 2 were positive for eae (1.2%) and efa1 (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for ehaA (98%), iha (97%), efa1 (95%), and eae (95%), respectively, while 11, 2, 2, and 1 were positive for lpfA(O113) (18%), saa (3.3%), cdt-V (3.3%), and subAB (1.6%), respectively. Therefore, in human STEC isolates efa1 and eae whereas in cattle isolates saa, lpfA(O113), cdt-V and subAB were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.
DOI：
10.1073/pnas.92.5.1664
发表时间：
1995-02-28
影响因子：
11.1
作者：
MCDANIEL, TK;JARVIS, KG;KAPER, JB
通讯作者：
KAPER, JB