Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments.
Considerations on Experimental Design and Data Analysis of Chromatin Immunoprecipitation Experiments.
复制标题
染色质免疫沉淀实验的实验设计和数据分析的考虑。
作者：
A. Jordán;N. Visa
ChIP 可以说是研究染色质组织最有价值的技术之一，是绘制蛋白质与基因组 DNA 之间建立的接触图谱的首选方法。自 30 多年前成立以来，ChIP 一直在不断发展、改进和扩大其能力和范围。尽管 ChIP 检测被许多跨学科的实验室广泛使用，但有时设计起来具有挑战性，并且通常对实际实施中的变化很敏感。在本章中，我们对 ChIP 方法及其最常见的变化进行总体概述，特别关注 ChIP-seq。我们试图解决一些需要考虑的最重要的方面，以便设计和执行生成最可重复、高质量数据的实验。涵盖的一些主要主题包括使用正确表征的抗体、染色质制备的替代方案、适当控制的需要以及有关 ChIP-seq 数据分析的一些建议。
Arguably one of the most valuable techniques to study chromatin organization, ChIP is the method of choice to map the contacts established between proteins and genomic DNA. Ever since its inception, more than 30 years ago, ChIP has been constantly evolving, improving, and expanding its capabilities and reach. Despite its widespread use by many laboratories across a wide variety of disciplines, ChIP assays can be sometimes challenging to design, and are often sensitive to variations in practical implementation.In this chapter, we provide a general overview of the ChIP method and its most common variations, with a special focus on ChIP-seq. We try to address some of the most important aspects that need to be taken into account in order to design and perform experiments that generate the most reproducible, high-quality data. Some of the main topics covered include the use of properly characterized antibodies, alternatives to chromatin preparation, the need for proper controls, and some recommendations about ChIP-seq data analysis.
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DOI：
10.1007/978-1-61779-376-9_6
发表时间：
2012
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Gade, Padmaja;Kalvakolanu, Dhan V.
通讯作者：
Kalvakolanu, Dhan V.
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK
影响因子：
16.8
作者：
Mayer, Andreas;Lidschreiber, Michael;Cramer, Patrick
通讯作者：
Cramer, Patrick
影响因子：
16
作者：
van Galen P;Viny AD;Ram O;Ryan RJ;Cotton MJ;Donohue L;Sievers C;Drier Y;Liau BB;Gillespie SM;Carroll KM;Cross MB;Levine RL;Bernstein BE
通讯作者：
Bernstein BE
DOI：
10.1073/pnas.81.14.4275
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GILMOUR, DS;LIS, JT
通讯作者：
LIS, JT