Genome organization, phylogenies, expression patterns, and three-dimensional protein models of two acetylcholinesterase genes from the red flour beetle.
Genome organization, phylogenies, expression patterns, and three-dimensional protein models of two acetylcholinesterase genes from the red flour beetle.
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DOI：
10.1371/journal.pone.0032288
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhu KY
中科院分区：
文献类型：
--
作者：
Lu Y;Pang YP;Park Y;Gao X;Yao J;Zhang X;Zhu KY
自2002年在绿蝽（Schizaphis graminum）中发现一个同源乙酰胆碱酯酶（AChE, EC3.1.1.7）基因以来，在至少27种昆虫中分别鉴定出两个不同的AChE基因（Ace1和Ace2）。然而，Ace1和Ace2的基因模型及其分子特性尚未在任何一种昆虫中得到全面分析。在这项研究中，我们对全球分布的主要储粮害虫和新兴模式生物——赤粉甲虫（Tribolium castaneum； TcAce1和TcAce2）的全长cdna进行了测序，计算预测了相应的三维蛋白质模型，并对两个Ace基因的发育阶段和组织特异性表达模式进行了分析。TcAce1和TcAce2分别编码648和604个氨基酸残基，具有保守的基序，包括胆碱结合位点、催化三联体和酰基口袋。系统发育分析表明，这两个基因都属于两个昆虫Ace基因簇，并且TcAce1与TcAce2完全分化，表明这两个基因在昆虫物种中是从对应的Ace基因谱系进化而来的。此外，TcAce1位于5号染色体上，而TcAce2位于2号染色体上。逆转录聚合酶链反应（PCR）和实时荧光定量PCR分析表明，这两个基因几乎在昆虫发育的所有阶段都有转录，并且主要在昆虫大脑中表达。我们的计算分析表明，TcAce1蛋白是一个强大的乙酰胆碱（ACh）水解酶，对巯基药物敏感，而TcAce2蛋白不是一个催化效率高的乙酰胆碱水解酶。
Since the report of a paralogous acetylcholinesterase (AChE, EC3.1.1.7) gene in the greenbug (Schizaphis graminum) in 2002, two different AChE genes (Ace1 and Ace2) have been identified in each of at least 27 insect species. However, the gene models of Ace1 and Ace2, and their molecular properties have not yet been comprehensively analyzed in any insect species. In this study, we sequenced the full-length cDNAs, computationally predicted the corresponding three-dimensional protein models, and profiled developmental stage and tissue-specific expression patterns of two Ace genes from the red flour beetle (Tribolium castaneum; TcAce1 and TcAce2), a globally distributed major pest of stored grain products and an emerging model organism. TcAce1 and TcAce2 encode 648 and 604 amino acid residues, respectively, and have conserved motifs including a choline-binding site, a catalytic triad, and an acyl pocket. Phylogenetic analysis show that both TcAce genes are grouped into two insect Ace clusters and TcAce1 is completely diverged from TcAce2, suggesting that these two genes evolve from their corresponding Ace gene lineages in insect species. In addition, TcAce1 is located on chromosome 5, whereas TcAce2 is located on chromosome 2. Reverse transcription polymerase chain reaction (PCR) and quantitative real-time PCR analyses indicate that both genes are virtually transcribed in all the developmental stages and predominately expressed in the insect brain. Our computational analyses suggest that the TcAce1 protein is a robust acetylcholine (ACh) hydrolase and has susceptibility to sulfhydryl agents whereas the TcAce2 protein is not a catalytically efficient ACh hydrolase.
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影响因子：
14.9
作者：
Felder CE;Prilusky J;Silman I;Sussman JL
通讯作者：
Sussman JL
影响因子：
4.4
作者：
JORGENSEN, WL;CHANDRASEKHAR, J;KLEIN, ML
通讯作者：
KLEIN, ML
DOI：
10.1093/protein/11.6.415
发表时间：
1998-06-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Botti, SA;Felder, CE;Silman, I
通讯作者：
Silman, I
影响因子：
8
作者：
Harel, M;Kryger, G;Sussman, JL
通讯作者：
Sussman, JL
ACE2（而非 ACE1）是家蚕 (Bombyx mori) 中主要的乙酰胆碱酯酶
影响因子：
4
作者：
Chen, Hui-Juan;Liao, Zhen;Han, Zhao-Jun
通讯作者：
Han, Zhao-Jun