A comprehensive sequence and disease correlation analyses for the C-terminal region of CagA protein of Helicobacter pylori.
A comprehensive sequence and disease correlation analyses for the C-terminal region of CagA protein of Helicobacter pylori.
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DOI：
10.1371/journal.pone.0007736
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发表时间：
2009-11-06
期刊：
影响因子：
3.7
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Xia Y;Yamaoka Y;Zhu Q;Matha I;Gao X
已知慢性幽门螺杆菌感染与消化性溃疡、胃癌和胃淋巴瘤的发生有关。目前，据报道幽门螺杆菌的细菌因素在胃十二指肠疾病的发生中起重要作用。由cagA编码的CagA蛋白是研究最深入的幽门螺杆菌毒力因子。致病性 CagA 蛋白的 C 末端含有高度多态性的 Glu-Pro-Ile-Tyr-Ala (EPIYA) 重复区域。据报道，该重复区域参与胃十二指肠疾病的发病机制。含有 EPIYA 基序的片段被指定为片段 A、B、C 和 D；然而其分类和疾病关系仍不清楚。这项研究使用了 560 个独特的 CagA 序列，其中包含从公共资源收集的 1,796 个 EPIYA 基序，其中包括 274 个西方菌株和 286 个东亚菌株，以及从 433 个条目中获得的临床数据。定义了十五种类型的 EPIYA 或 EPIYA 类序列。除了之前报道的四种主要片段类型之外，还使用我们的分类方法定义了几种次要片段类型（例如片段 B'、B''）和 30 多种序列类型（例如 ABC、ABD）。我们确认来自西方和东亚菌株的序列分别包含 C 和 D 片段。我们还证实，具有两个 EPIYA C 片段的菌株比具有一个 C 片段的菌株患胃癌的机会更大。我们的结果揭示了 CagAs 类型、每种序列类型的原产国和胃病频率之间的关系。
Chronic Helicobacter pylori infection is known to be associated with the development of peptic ulcer, gastric cancer and gastric lymphoma. Currently, the bacterial factors of H. pylori are reported to be important in the development of gastroduodenal diseases. CagA protein, encoded by the cagA, is the best studied virulence factor of H. pylori. The pathogenic CagA protein contains a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal. This repeat region is reported to be involved in the pathogenesis of gastroduodenal diseases. The segments containing EPIYA motifs have been designated as segments A, B, C, and D; however the classification and disease relation are still unclear. This study used 560 unique CagA sequences containing 1,796 EPIYA motifs collected from public resources, including 274 Western and 286 East Asian strains with clinical data obtained from 433 entries. Fifteen types of EPIYA or EPIYA-like sequences are defined. In addition to four previously reported major segment types, several minor segment types (e.g., segment B′, B′′) and more than 30 sequence types (e.g., ABC, ABD) were defined using our classification method. We confirm that the sequences from Western and East Asian strains contain segment C and D, respectively. We also confirm that strains with two EPIYA segment C have a greater chance of developing gastric cancer than those with one segment C. Our results shed light on the relationships between the types of CagAs, the country of origin of each sequence type, and the frequency of gastric disease.
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影响因子：
3.4
作者：
Backert, S;Ziska, E;Meyer, TF
通讯作者：
Meyer, TF
影响因子：
29.4
作者：
Argent, RH;Kidd, M;Atherton, JC
通讯作者：
Atherton, JC
DOI：
10.1073/pnas.90.12.5791
发表时间：
1993-06-15
影响因子：
11.1
作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
通讯作者：
RAPPUOLI, R
影响因子：
4.8
作者：
Higashi, H;Yokoyama, K;Hatakeyama, M
通讯作者：
Hatakeyama, M
DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A