The etiologic role of human papillomavirus in penile cancers: a study in Vietnam.
The etiologic role of human papillomavirus in penile cancers: a study in Vietnam.
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DOI：
10.1038/bjc.2012.583
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发表时间：
2013-01-15
影响因子：
8.8
通讯作者：
Akiba, S.
中科院分区：
文献类型：
--
作者：
Do, H. T. T.;Koriyama, C.;Khan, N. A.;Higashi, M.;Kato, T.;Le, N. T.;Matsushita, S.;Kanekura, T.;Akiba, S.
我们调查了越南120例阴茎鳞状细胞癌（PSCCs）中人乳头瘤病毒（HPV）的病因学作用。用SPF10引物和hpv - 16e6引物对人乳头瘤病毒DNA进行PCR检测。采用ino - lipa HPV基因分型试剂盒进行基因型检测。实时荧光定量PCR检测人乳头瘤病毒-16病毒载量及生理状态。免疫组织化学检测P16INK4A蛋白表达。120例PSCCs中有27例（23%）检测到人乳头瘤病毒DNA。最常检测到的基因型是HPV-16（27例中有24例，89%）。在18例（89%）HPV-16阳性病例中的16例中，HPV DNA被认为整合到宿主基因组中。HPV-16病毒载量的几何平均值为每个细胞0.4个拷贝。P16INK4A过表达与PSCCs感染高危HPV （P=0.018）和HPV-16拷贝数（P<0.001）显著相关。人乳头瘤病毒-16 DNA整合和p16INK4A在高危HPV检测到的PSCCs中的过表达提示高危HPV在PSCCs发展中的病因学作用。
We investigated the aetiologic role of human papillomavirus (HPV) in 120 penile squamous cell carcinomas (PSCCs) from Vietnam. Human papillomavirus DNA was detected by PCR using SPF10 primers and a primer set targeting HPV-16 E6. The INNO-LiPA HPV genotyping kit was used to determine genotype. Human papillomavirus-16 viral load and physical status were determined by real-time PCR. P16INK4A protein expression was investigated by immunohistochemistry. Human papillomavirus DNA was detected in 27 of 120 (23%) PSCCs. The most frequently detected genotype was HPV-16 (24 of 27 cases, 89%). In 16 of 18 (89%) HPV-16-positive cases, the HPV DNA was considered to be integrated into the host genome. The geometric mean of the HPV-16 viral load was 0.4 copies per cell. P16INK4A overexpression was significantly related to PSCCs infected with high-risk HPV (P=0.018) and HPV-16 copy numbers (P<0.001). Human papillomavirus-16 DNA integration and p16INK4A overexpression in high-risk HPV detected PSCCs suggested an aetiologic role of high-risk HPV in the development of PSCCs.
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