Recent amplification and impact of MITEs on the genome of grapevine (Vitis vinifera L.).
Recent amplification and impact of MITEs on the genome of grapevine (Vitis vinifera L.).
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DOI：
10.1093/gbe/evp009
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发表时间：
2009-05-20
影响因子：
3.3
通讯作者：
Casacuberta JM
中科院分区：
文献类型：
--
作者：
Benjak A;Boué S;Forneck A;Casacuberta JM
微型反向重复转座元件(MITE)是一种特殊类型的缺陷II类转座子，以高度均一的小元件种群存在于基因组中。它们的高拷贝数和与基因的密切联系使得它们对基因进化的潜在影响特别相关。在这里，我们提出了一个与葡萄“剪切粘贴”转座子直接相关的螨类家族的详细分析。我们的结果表明，葡萄叶螨具有反式复制和扩增的基因组序列，包括基因序列和其他可移动元件的片段。我们的结果还表明，虽然在欧美葡萄野生种的祖先中已经存在一些螨科，但它们已经被扩增，并积极地转座伴随着葡萄的驯化和繁殖。我们发现，在葡萄中有大量的螨类，其中一些经常插入到葡萄基因的非翻译区。在葡萄品种中，螨类插入是高度多态的，这经常产生转录本的变异。这里提供的数据表明，螨类对葡萄的遗传多样性做出了巨大贡献，这种遗传多样性已被用于葡萄的驯化和育种。
Miniature inverted-repeat transposable elements (MITEs) are a particular type of defective class II transposons present in genomes as highly homogeneous populations of small elements. Their high copy number and close association to genes make their potential impact on gene evolution particularly relevant. Here, we present a detailed analysis of the MITE families directly related to grapevine “cut-and-paste” transposons. Our results show that grapevine MITEs have transduplicated and amplified genomic sequences, including gene sequences and fragments of other mobile elements. Our results also show that although some of the MITE families were already present in the ancestor of the European and American Vitis wild species, they have been amplified and have been actively transposing accompanying grapevine domestication and breeding. We show that MITEs are abundant in grapevine and some of them are frequently inserted within the untranslated regions of grapevine genes. MITE insertions are highly polymorphic among grapevine cultivars, which frequently generate transcript variability. The data presented here show that MITEs have greatly contributed to the grapevine genetic diversity which has been used for grapevine domestication and breeding.
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影响因子：
30.8
作者：
Morgante, M;Brunner, S;Rafalski, A
通讯作者：
Rafalski, A
影响因子：
4.9
作者：
Arroyo-Garcia, R.;Ruiz-Garcia, L.;Martinez-Zapater, J. M.
通讯作者：
Martinez-Zapater, J. M.
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
DOI：
10.1073/pnas.0502923102
发表时间：
2005-06-21
影响因子：
11.1
作者：
Lai, JS;Li, YB;Dooner, HK
通讯作者：
Dooner, HK
来自微型倒重复转座元件的人类microRNA基因家族。
影响因子：
3.7
作者：
Piriyapongsa, Jittima;Jordan, I. King
通讯作者：
Jordan, I. King