Simultaneous radial and wavelength analysis with the Optima XL-A analytical ultracentrifuge
Simultaneous radial and wavelength analysis with the Optima XL-A analytical ultracentrifuge
复制标题
使用 Optima XL-A 分析超速离心机同时进行径向和波长分析
DOI:
10.1007/bfb0115597
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发表时间:
1994
影响因子:
--
通讯作者:
P. Schuck
中科院分区:
文献类型:
--
作者:
P. Schuck
We exploit the possibility offered by the new analytical ultracentrifuge Beckman Optima XL-A to record wavelength profiles at different radial positions and radial profiles at different wavelengths in sedimentation equilibrium experiments to unravel complexes formed by entities with different absorption spectra. The mathematical analysis is described here in detail. With a little a priori knowledge about the absorption spectra it is possible to resolve a greater number of components than with conventional methods. This method also enables one to achieve a simultaneous detection of the components’ extinction profiles. In addition, an analysis of each component’s statistical accuracy is presented.
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影响因子:
--
作者:
J. M. Beechem
通讯作者:
J. M. Beechem
影响因子:
--
作者:
M. L. Johnson;L. M. Faunt
通讯作者:
M. L. Johnson;L. M. Faunt
影响因子:
2.9
作者:
Laue,TM;Senear,DF;Eaton,S;Ross,JB
通讯作者:
Ross,JB
影响因子:
3.4
作者:
JOHNSON, ML;CORREIA, JJ;HALVORSON, HR
通讯作者:
HALVORSON, HR