An Endophytic Taxol-Producing Fungus from Taxus media, Cladosporium cladosporioides MD2
An Endophytic Taxol-Producing Fungus from Taxus media, Cladosporium cladosporioides MD2
复制标题
红豆杉属内生紫杉醇生产真菌 Cladosporium cladosporioides MD2
DOI：
10.1007/s00284-008-9270-1
复制
发表时间：
2009-05
影响因子：
2.6
通讯作者：
Zhang, Peng
中科院分区：
文献类型：
--
作者：
Zhou, Peng-Peng;Yu, Long-Jiang;Zhang, Peng
紫杉醇是临床上广泛应用的重要抗肿瘤药物，利用红豆杉属以外的产紫杉醇真菌进行发酵是生产紫杉醇的另一种途径。本研究从红豆杉（Taxus media）的内树皮中分离到一株产紫杉醇内生真菌MD2。菌株MD2在马铃薯葡萄糖液体培养基中生长时产生紫杉醇。经紫外、高效液相色谱和质谱分析，证实该真菌紫杉醇与真品紫杉醇和10-去乙酰巴accatin III相同。进一步的核磁共振波谱分析表明，真菌紫杉醇的化学结构与真品紫杉醇的核磁共振波谱一致。菌株MD2根据真菌培养形态、孢子特征及18S rDNA序列分析鉴定为Cladosporium cladosporioides。此外，首次克隆出了cladspororioides MD2的10- deacetylbacaccatin iii -10- o - acetyltransferase基因，该基因与T. x和T. wallichiana var.marei的同源性分别为99%和97%。
Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media. Strain MD2 produced taxol when grown in potato dextrose liquid medium. The fungal taxol-which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry-was shown to be identical to authentic taxol and 10-deacetylbaccatin III. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal taxol indicated that the fungal taxol produced an NMR spectrum identical to that of authentic taxol. Strain MD2 was identified as Cladosporium cladosporioides according to morphology of the fungal culture, characteristics of the spores, and analysis of 18S rDNA sequence. In addition, 10-deacetylbaccatin III-10-O-acetyl transferase gene of C. cladosporioides MD2 was cloned for the first time and was shown to share 99% identity with that of T. x media and 97% identity with that of T. wallichiana var. mairei.
登录
查看更多内容
影响因子：
1.5
作者：
Y. Sergeeva;I. Konova;L. A. Galanina;A. B. Gagarina;N. M. Evteeva
通讯作者：
Y. Sergeeva;I. Konova;L. A. Galanina;A. B. Gagarina;N. M. Evteeva
影响因子：
15
作者：
WANI, MC;TAYLOR, HL;MCPHAIL, AT
通讯作者：
MCPHAIL, AT
DOI：
10.1099/13500872-142-8-2223
发表时间：
1996-08-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Li, JY;Strobel, G;Ford, EJ
通讯作者：
Ford, EJ
影响因子：
1.5
作者：
B. Daunter;R. N. Greenshields
通讯作者：
B. Daunter;R. N. Greenshields
影响因子：
2.1
作者：
Jianfeng Wang;Guiling Li;Huaying Lu;Z. Zheng;Yaojian Huang;Wen-jin Su
通讯作者：
Jianfeng Wang;Guiling Li;Huaying Lu;Z. Zheng;Yaojian Huang;Wen-jin Su