Detection of brain-directed autoantibodies in the serum of non-small cell lung cancer patients.
Detection of brain-directed autoantibodies in the serum of non-small cell lung cancer patients.
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DOI：
10.1371/journal.pone.0181409
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Janigro D
中科院分区：
文献类型：
--
作者：
Banjara M;Ghosh C;Dadas A;Mazzone P;Janigro D
在癌症患者的血浆中发现了针对脑蛋白的抗体，并被确定为引起副肿瘤神经综合征。非小细胞肺癌（NSCLC）中脑定向抗体的概况在很大程度上是未知的。在这里，我们首次比较了非小细胞肺癌（n = 18）与年龄匹配的非癌症对照（n = 18）中存在的针对脑蛋白的自身抗体，这些受试者具有相似的生活方式、习惯和病史。自我识别免疫球蛋白（IgG）主要针对皮层（P = 0.008）、海马（P = 0.003-0.05）和小脑（P = 0.02）的细胞。更具体地说，IgG靶点在金字塔细胞层、浦肯野细胞层和颗粒细胞层中都很突出。此外，自身免疫IgG信号定位于神经元（81%）、星形胶质细胞（48%）和内皮细胞（29%）。虽然癌症血清产生的信号强度总体较高，但分子量为100、65、45、37和30 kDa的自身抗原最具代表性。此外，一组100 kDa蛋白似乎在女性腺癌患者中更为普遍（4/ 5,80 %）。总之，我们的研究结果揭示了NSCLC的自身抗原特异性，这隐含地取决于患者的人口统计学和病史。有肺癌风险但无活动性疾病的患者表明，非小细胞肺癌的免疫谱是疾病依赖性的。
Antibodies against brain proteins were identified in the plasma of cancer patients and are defined to cause paraneoplastic neurological syndromes. The profiles of brain-directed antibodies in non-small cell lung cancer (NSCLC) are largely unknown. Here, for the first time, we compared autoantibodies against brain proteins in NSCLC (n = 18) against those present in age-matched non-cancer control subjects (n = 18) with a similar life-style, habit, and medical history. Self-recognizing immunoglobulin (IgG) are primarily directed against cells in the cortex (P = 0.008), hippocampus (P = 0.003–0.05), and cerebellum (P = 0.02). More specifically, IgG targets were prominent in the pyramidal, Purkinje, and granule cell layers. Furthermore, autoimmune IgG signals were localized to neurons (81%), astrocytes (48%), and endothelial (29%) cells. While cancer sera yielded overall higher intensity signals, autoantigens of 100, 65, 45, 37, and 30 kDa molecular weights were the most represented. Additionally, a group of 100 kDa proteins seem more prevalent in female adenocarcinoma patients (4/5, 80%). In conclusion, our results revealed autoantigen specificity in NSCLC, which implicitly depends on patient’s demographics and disease history. Patients at risk for lung cancer but with no active disease revealed that the immune profile in NSCLC is disease-dependent.
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