Regulation of Cell Polarity by Exocyst-Mediated Trafficking.
Regulation of Cell Polarity by Exocyst-Mediated Trafficking.
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DOI：
10.1101/cshperspect.a031401
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发表时间：
2018-03-01
影响因子：
7.2
通讯作者：
Fogelgren B
中科院分区：
文献类型：
--
作者：
Polgar N;Fogelgren B
在细胞内建立极性的一个要求是细胞内囊泡向质膜的不对称运输。这种严格调节的过程在质膜组成和膜相关蛋白质组中产生了空间和时间差异。不对称膜运输也是调节细胞分化、信号传导和生理学的关键机制。许多真核细胞类型使用八蛋白外囊复合物来调节极化囊泡运输到某些膜区域。外囊的成员最初是在酵母中发现的，同时筛选将分泌囊泡递送到出芽子细胞所需的蛋白质。同样的八个外囊基因在哺乳动物中是保守的，其中外囊介导的运输的特异性是高度依赖于细胞类型的。一些胞外囊成员与胞内囊泡上的某些Rab GTP酶结合，而另一些则定位于胞吐部位的质膜上。外泌囊全复合物的组装负责在它们的SNARE介导的胞吐作用之前将这些囊泡拴系到质膜。在这篇综述中，我们将重点介绍与细胞极性的建立和维持相关的外囊复合物在靶向囊泡运输中的作用和调控。我们将对比顶-基底上皮极性与前-后间充质极性中的外囊功能，以及细胞表型转变期间外囊介导的贩运的动态调节。
One requirement for establishing polarity within a cell is the asymmetric trafficking of intracellular vesicles to the plasma membrane. This tightly-regulated process creates spatial and temporal differences in both plasma membrane composition and the membrane-associated proteome. Asymmetric membrane trafficking is also a critical mechanism to regulate cell differentiation, signaling, and physiology. Many eukaryotic cell types use the eight-protein exocyst complex to regulate polarized vesicle trafficking to certain membrane locales. Members of the exocyst were originally discovered in yeast while screening for proteins required for the delivery of secretory vesicles to the budding daughter cell. The same eight exocyst genes are conserved in mammals, where the specifics of exocyst-mediated trafficking are highly cell-type dependent. Some exocyst members bind to certain Rab GTPases on intracellular vesicles, while others localize to the plasma membrane at the site of exocytosis. Assembly of the exocyst holocomplex is responsible for tethering these vesicles to the plasma membrane prior to their SNARE-mediated exocytosis. In this review, we will focus on the role and regulation of the exocyst complex in targeted vesicular trafficking as related to the establishment and maintenance of cellular polarity. We will contrast exocyst function in apico-basal epithelial polarity versus front-back mesenchymal polarity, and the dynamic regulation of exocyst-mediated trafficking during cell phenotype transitions.
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影响因子：
7
作者：
Barile, M;Pisitkun, T;Knepper, MA
通讯作者：
Knepper, MA
影响因子：
7.8
作者：
Beronja, Slobodan;Laprise, Patrick;Papoulas, Ophelia;Pellikka, Milena;Sisson, John;Tepass, Ulrich
通讯作者：
Tepass, Ulrich
影响因子：
4
作者：
Das, Amlan;Gajendra, Sangeetha;Lalli, Giovanna
通讯作者：
Lalli, Giovanna
影响因子：
14.9
作者：
UniProt Consortium
通讯作者：
UniProt Consortium
影响因子：
3.7
作者：
Fogelgren B;Polgar N;Lui VH;Lee AJ;Tamashiro KK;Napoli JA;Walton CB;Zuo X;Lipschutz JH
通讯作者：
Lipschutz JH