Secreted sialidase activity of canine mycoplasmas.
Secreted sialidase activity of canine mycoplasmas.
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DOI：
10.1016/j.vetmic.2009.01.009
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发表时间：
2009-06-12
影响因子：
3.3
通讯作者：
Brown DR
中科院分区：
文献类型：
--
作者：
May M;Brown DR
通过调查的支原体之间的唾液酸酶的系统发育分布，我们检测的活性分泌的模式菌株的11个物种中的三个经常或首次从狗。犬支原体、犬支原体和噬菌支原体模式菌株的洗涤细胞比活性范围为5.2 ± 0.8 × 10-6至1.1 ± 0.3 × 10-5酶单位/菌落形成单位（U/CFU）。M.经测定，H542 T菌株的比活是M.犬支原体PG 14 T或M.食蟹猴菌株H831 T。9株M.犬感染，范围为2.1 ± 0.1 × 10-5 U/CFU。先前从犬中分离出的其他种属的模式菌株（支原体、牛生殖支原体、爱德华支原体、猫支原体、弓形支原体、斑点支原体、乳白支原体和泡沫支原体）未表现出分泌型或细胞相关唾液酸酶活性。与三个已知支原体唾液酸酶等位基因互补的特异性或简并PCR引物均不能扩增支原体的直系同源物。canis，M. cynos或M. Molare的结果，进一步证明了这些物种分泌的唾液酸酶不同于美洲鳄支原体、滑液支原体和鸡毒支原体的严格细胞相关的唾液酸酶。这是第一次报道一个众所周知的细菌毒力因子，其表达在感染犬的某些支原体属菌株中存在差异。
Through a survey of the phylogenetic distribution of sialidase among mycoplasmas, we detected activity secreted by the type strains of three of eleven species frequently or first isolated from dogs. The specific activity of washed cells of the type strains of Mycoplasma canis, Mycoplasma cynos, and Mycoplasma molare ranged from 5.2 ± 0.8 × 10-6 to 1.1 ± 0.3 × 10-5 enzymatic units per colony-forming unit (U/CFU). Cells of M. molare strain H542T had twice the specific activity (P < 0.05) of M. canis strain PG14T or M. cynos strain H831T. Significant differences in sialidase activity existed among nine clinical isolates of M. canis, ranging from not detectable to 2.1 ± 0.1 × 10-5 U/CFU. The type strains of other species previously isolated from dogs (Mycoplasma arginini, Mycoplasma bovigenitalium, Mycoplasma edwardii, Mycoplasma felis, Mycoplasma gatae, Mycoplasma maculosum, Mycoplasma opalescens, and Mycoplasma spumans) did not exhibit either secreted or cell-associated sialidase activity. Neither specific nor degenerate PCR primers complementary to the three known mycoplasmal sialidase alleles were able to amplify orthologs in M. canis, M. cynos, or M. molare, further evidence that the secreted sialidase of those species is distinct from the strictly cell-associated sialidases of Mycoplasma alligatoris, Mycoplasma synoviae, and Mycoplasma gallisepticum. This is the first report of a well-known bacterial virulence factor whose expression varies among strains of certain Mycoplasma species that infect dogs.
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DOI：
10.1099/0096266x-5-2-85
发表时间：
1955-01-01
期刊：
INTERNATIONAL BULLETIN OF BACTERIOLOGICAL NOMENCLATURE AND TAXONOMY
影响因子：
--
作者：
EDWARD, DG
通讯作者：
EDWARD, DG
影响因子：
2.8
作者：
LABER, G;HOLZMANN, A
通讯作者：
HOLZMANN, A
影响因子：
2.2
作者：
Ayling, RD;Bashiruddin, SE;Nicholas, RAJ
通讯作者：
Nicholas, RAJ
DOI：
10.1099/00207713-23-1-49
发表时间：
1973-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
ROSENDAL, S
通讯作者：
ROSENDAL, S
DOI：
10.1099/00207713-24-1-125
发表时间：
1974-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
ROSENDAL, S
通讯作者：
ROSENDAL, S