Effect of glucose on the biomechanical function of arterial elastin.
Effect of glucose on the biomechanical function of arterial elastin.
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DOI：
10.1016/j.jmbbm.2015.04.025
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发表时间：
2015-09
影响因子：
3.9
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Wang Y;Zeinali-Davarani S;Davis EC;Zhang Y
弹性蛋白是为血管提供弹性支撑所必需的。作为一种非常长寿的蛋白质，弹性蛋白可能会受到暴露于生化损伤的累积效应，这会极大地损害其生物力学特性。非酶糖基化是衰老的主要机制之一，其作用在糖尿病患者中被放大。本研究旨在探讨葡萄糖对猪主动脉弹性蛋白力学性质的影响。将弹性蛋白样品在2 M葡萄糖溶液中孵育，并使其在37°C下平衡4、7、14、21或28天。通过等双轴拉伸实验研究了糖基化对弹性蛋白弹性性能的影响。葡萄糖孵育7天后，观察到组织尺寸显著减小。处理14、21或28天的弹性蛋白样品在应力-拉伸曲线中表现出滞后的显著增加，表明由于葡萄糖处理导致的更大的能量损失。纵向和周向均显示出葡萄糖处理的切线模量显著增加，然而，仅在7天后观察到周向的显著增加。基于八链统计力学的微结构模型被用来研究葡萄糖处理的弹性蛋白的超弹性和正交各向异性行为，并且使用非线性最小二乘法估计材料参数。模型中的材料参数与弹性蛋白密度和纤维取向相关，因此也与葡萄糖处理的弹性蛋白中可能的微观结构变化相关。估计的材料参数显示出葡萄糖孵育的每单位体积的弹性蛋白密度的总体增加趋势。模拟结果还表明，更多的弹性纤维排列在纵向和周向方向，而不是在径向方向。
Elastin is essential to provide elastic support for blood vessels. As a remarkably long-lived protein, elastin can suffer from cumulative effects of exposure to biochemical damages, which can greatly compromise its biomechanical properties. Non-enzymatic glycation is one of the main mechanisms of aging and its effect is magnified in diabetic patients. The purpose of this study is to investigate the effects of glucose on mechanical properties of isolated porcine aortic elastin. Elastin samples were incubated in 2 M glucose solution and were allowed to equilibrate for 4, 7, 14, 21 or 28 days at 37°C. Equibiaxial tensile tests were performed to study the changes of elastic properties of elastin due to glycation. Significant decreases in tissue dimension were observed after 7 days glucose incubation. Elastin samples treated for 14, 21 or 28 days demonstrate a significant increase in hysteresis in the stress-stretch curves, indicating a greater energy loss due to glucose treatment. Both the longitudinal and the circumferential directions show significant increases in tangent modulus with glucose treatment, however only significant increases are observed after 7 days for the circumferential direction. An eight-chain statistical mechanics based microstructural model was used to study the hyperelastic and orthotropic behavior of the glucose-treated elastin and the material parameters were estimated using a nonlinear least squares method. Material parameters in the model were related to elastin density and fiber orientation, and, hence, the possible microstructural changes in glucose-treated elastin. Estimated material parameters show a general increasing trend in elastin density per unit volume with glucose incubation. The simulation results also indicate that more elastic fibers are aligned in the longitudinal and circumferential directions, rather than in the radial direction.
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影响因子：
5.3
作者：
CAMPA, JS;GREENHALGH, RM;POWELL, JT
通讯作者：
POWELL, JT
影响因子：
2.9
作者：
GOSLINE, JM;FRENCH, CJ
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FRENCH, CJ
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作者：
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作者：
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