Dissemination of bla(NDM-5) gene via an IncX3-type plasmid among non-clonal Escherichia coli in China.
Dissemination of bla(NDM-5) gene via an IncX3-type plasmid among non-clonal Escherichia coli in China.
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bla(NDM-5)基因通过IncX3型质粒在中国非克隆大肠杆菌中的传播
DOI：
10.1186/s13756-018-0349-6
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发表时间：
2018
影响因子：
5.5
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Li X;Fu Y;Shen M;Huang D;Du X;Hu Q;Zhou Y;Wang D;Yu Y
由于其多重耐药性在全球范围内迅速传播，产生新德里金属-β-内酰胺酶的肠杆菌科细菌的出现和传播一直是临床管理的严峻挑战。作为碳青霉烯酶的主要类型之一，新德里金属-β-内酰胺酶（NDM）能够对肠杆菌科细菌中的几乎所有β-内酰胺类（包括碳青霉烯类）产生耐药性。最近，新德里金属-β-内酰胺酶-5因碳青霉烯类耐药性增加且广泛传播而引起广泛关注。然而，blaNDM-5基因的传播机制仍不清楚。从浙江省不同医院收集了224株耐碳青霉烯类肠杆菌科细菌(CRE)。鉴定出 NDM-5 阳性分离株并进行基因分型、药敏试验和临床数据分析。我们通过 Southern blot 杂交确定了 blaNDM-5 的遗传位置，并通过滤膜交配和 DNA 测序分析了含有 blaNDM-5 的质粒。鉴定出11株新德里金属β-内酰胺酶5（NDM-5）产生菌株，其中大肠杆菌9株、肺炎克雷伯菌1株、弗氏柠檬酸杆菌1株。通过多位点序列分型 (MLST) 和脉冲场凝胶电泳 (PFGE) 未发现大肠杆菌分离株的流行病学联系。 S1-PFGE 和 Southern blot 表明 blaNDM-5 基因位于所有分离株中的 46 kb IncX3 型质粒上。测试的 11 个分离株中有 9 个（81.8%）能够成功地将其碳青霉烯类耐药表型转移到大肠杆菌菌株 C600 中。此外，序列分析进一步表明，该质粒与国内报道的大多数携带blaNDM-5的质粒具有高度的序列相似性。本研究中的现有数据表明 IncX3 型质粒在 blaNDM-5 在肠杆菌科细菌中的传播中发挥重要作用。此外，据我们所知，该报告首次从一名患者身上分离出携带blaNDM-5的大肠杆菌和弗氏梭菌菌株，这进一步表明了blaNDM-5在不同物种之间传播的可能性。迫切需要密切监测以监测产生 NDM-5 的分离株的进一步传播。本文的在线版本 (10.1186/s13756-018-0349-6) 包含补充材料，可供授权用户使用。
The emergence and spread of New Delhi metallo-β-lactamase-producing Enterobacteriaceae has been a serious challenge to manage in the clinic due to its rapid dissemination of multi-drug resistance worldwide. As one main type of carbapenemases, New Delhi metallo-β-lactamase (NDM)is able to confer resistance to almost all β-lactams, including carbapenems, in Enterobacteriaceae. Recently, New Delhi metallo-β-lactamase-5 attracted extensive attention because of increased resistance to carbapenems and widespread dissemination. However, the dissemination mechanism of blaNDM-5 gene remains unclear. A total of 224 carbapenem-resistant Enterobacteriaceae isolates (CRE) were collected from different hospitals in Zhejiang province. NDM-5-positive isolates were identified and subjected to genotyping, susceptibility testing, and clinical data analysis. We established the genetic location of blaNDM-5 with southern blot hybridisation, and analysed plasmids containing blaNDM-5 with filter mating and DNA sequencing. Eleven New Delhi metallo-β-lactamase-5 (NDM-5)-producing strains were identified, including 9 Escherichia coli strains, 1 Klebsiella pneumoniae strain, and 1 Citrobacter freundii strain. No epidemiological links for E. coli isolates were identified by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). S1-PFGE and southern blot suggested that the blaNDM-5 gene was located on a 46-kb IncX3-type plasmid in all isolates. Nine of the 11 isolates (81.8%) tested could successfully transfer their carbapenem-resistant phenotype to E. coli strain C600. Moreover, sequence analysis further showed that this plasmid possessed high sequence similarity to most of previously reported blaNDM-5-habouring plasmids in China. The present data in this study showed the IncX3 type plasmid played an important role in the dissemination of blaNDM-5 in Enterobacteriaceae. In addition, to the best of our knowledge, this report is the first to isolate both E. coli and C. freundii strains carrying blaNDM-5 from one single patient, which further indicated the possibility of blaNDM-5 transmission among diverse species. Close surveillance is urgently needed to monitor the further dissemination of NDM-5-producing isolates. The online version of this article (10.1186/s13756-018-0349-6) contains supplementary material, which is available to authorized users.
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影响因子：
5.2
作者：
Baraniak, A.;Izdebski, R.;Gniadkowski, M.
通讯作者：
Gniadkowski, M.
影响因子：
1.6
作者：
Krishnaraju, M.;Kamatchi, C.;Vaidyanathan, R.
通讯作者：
Vaidyanathan, R.
影响因子：
4.9
作者：
Hornsey, Michael;Phee, Lynette;Wareham, David W.
通讯作者：
Wareham, David W.
影响因子：
14.9
作者：
Grant JR;Stothard P
通讯作者：
Stothard P
影响因子：
6.4
作者：
Bulman ZP;Chen L;Walsh TJ;Satlin MJ;Qian Y;Bulitta JB;Peloquin CA;Holden PN;Nation RL;Li J;Kreiswirth BN;Tsuji BT
通讯作者：
Tsuji BT