XoxF encoding an alternative methanol dehydrogenase is widespread in coastal marine environments.
XoxF encoding an alternative methanol dehydrogenase is widespread in coastal marine environments.
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XoxF 编码另一种甲醇脱氢酶，广泛存在于沿海海洋环境中。
DOI：
10.1111/1462-2920.12896
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发表时间：
2015
影响因子：
5.1
通讯作者：
J. Murrell
中科院分区：
文献类型：
--
作者：
Martin Taubert;Martin Taubert;C. Grob;Alexandra M. Howat;Oliver J. Burns;J. Dixon;Yin Chen;J. Murrell
xoxF基因编码一种吡咯喹啉醌依赖的甲醇脱氢酶，在所有已知的甲基营养蛋白细菌中都有发现。在一些新发现的甲基营养物中，XoxF是活性甲醇脱氢酶，催化甲醇氧化成甲醛。除此之外，它在甲基化和碳循环中的潜在作用尚不清楚。到目前为止，环境中xoxF的多样性很少受到关注。我们设计了针对xoxF基因分支的PCR引物，并对从四个沿海海洋环境中获得的PCR扩增子进行了454焦磷酸测序，以独特的方式评估这些栖息地中xoxF的多样性。系统发育分析显示，两个研究分支的xoxF基因具有高度多样性，并且在不同环境下序列组成存在显著差异。这些序列被归类为与来自Alphaproteobacteria、Betaproteobacteria和Gammaproteobacteria的广泛的甲基营养体和非甲基营养体相关。检测到的最突出的序列与Rhodobacteraceae家族、Methylotenera属和Methylophilales的OM43分支有关，因此与利用XoxF进行甲醇氧化的生物有关。此外，我们的分析揭示了迄今为止未描述的高度序列，表明这些栖息地中存在大量未知的细菌物种。
The xoxF gene, encoding a pyrroloquinoline quinone-dependent methanol dehydrogenase, is found in all known proteobacterial methylotrophs. In several newly discovered methylotrophs, XoxF is the active methanol dehydrogenase, catalysing the oxidation of methanol to formaldehyde. Apart from that, its potential role in methylotrophy and carbon cycling is unknown. So far, the diversity of xoxF in the environment has received little attention. We designed PCR primer sets targeting clades of the xoxF gene, and used 454 pyrosequencing of PCR amplicons obtained from the DNA of four coastal marine environments for a unique assessment of the diversity of xoxF in these habitats. Phylogenetic analysis of the data obtained revealed a high diversity of xoxF genes from two of the investigated clades, and substantial differences in sequence composition between environments. Sequences were classified as being related to a wide range of both methylotrophs and non-methylotrophs from Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria. The most prominent sequences detected were related to the family Rhodobacteraceae, the genus Methylotenera and the OM43 clade of Methylophilales, and are thus related to organisms that employ XoxF for methanol oxidation. Furthermore, our analyses revealed a high degree of so far undescribed sequences, suggesting a high number of unknown bacterial species in these habitats.
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影响因子：
2.9
作者：
Barber, RD;Donohue, TJ
通讯作者：
Donohue, TJ
影响因子：
10.5
作者：
Chistoserdova L;Kalyuzhnaya MG;Lidstrom ME
通讯作者：
Lidstrom ME
影响因子：
2.1
作者：
Boden，Rich;Murrell，J. Colin;Schaefer，Hendrik
通讯作者：
Schaefer，Hendrik
影响因子：
4.9
作者：
Dixon, J. L.;Beale, R.;Nightingale, P. D.
通讯作者：
Nightingale, P. D.