Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a 'gold standard'.
Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a 'gold standard'.
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DOI：
10.1016/j.ijpara.2009.09.003
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发表时间：
2010-03-15
影响因子：
4
通讯作者：
McGarvey ST
中科院分区：
文献类型：
--
作者：
Tarafder MR;Carabin H;Joseph L;Balolong E Jr;Olveda R;McGarvey ST
Kato-Katz 技术在识别土源性蠕虫 (STH) 感染个体方面的准确性受到蠕虫卵排泄的日常变化、与其他寄生虫的混淆以及实验室技术人员的经验的限制。我们的目的是在缺乏“金标准”的情况下，使用贝叶斯方法评估 Kato-Katz 技术检测蛔虫、钩虫和鞭虫感染的敏感性和特异性。数据取自 2004 年 1 月至 2005 年 12 月在菲律宾萨马省进行的一项纵向研究。每个参与者连续几天提供一到三个粪便样本。使用 Kato-Katz 技术检查粪便样本，并报告 STH 阳性或阴性。在存在测量误差的情况下，每个人的真实状态被视为潜在数据。使用贝叶斯方法，我们根据观察数据和潜在数据的似然函数的乘积计算了敏感性和特异性参数的边际后验密度。使用均匀先验分布（β 分布：α=1，β=1）。共有 5,624 人提供了至少一份粪便样本。 1,582 人、1,893 人和 2,149 人分别提供了一份、两份和三份粪便样本。所有 STH 的测试结果每天都有变化。 Kato-Katz 技术对一份粪便样本的敏感性估计为 96.9%（95% 贝叶斯可信区间 [BCI]：96.1%、97.6%）、65.2%（60.0%、69.8%）和 91.4%（90.5%、92.3%），对于线虫、钩虫和毛线虫，分别。一份粪便样本对蚓状线虫、钩虫和毛线虫的特异性估计分别为 96.1%（95.5%、96.7%）、93.8%（92.4%、95.4%）和 94.4%（93.2%、95.5%）。我们的结果表明，Kato-Katz 技术对于 A. lumbricoides 和 T. trichiura 一天的粪便采集具有相当的准确性。 Kato-Katz 检测钩虫感染的低灵敏度可能与脆弱的钩虫卵随时间的快速退化有关。
The accuracy of the Kato-Katz technique in identifying individuals with soil-transmitted helminth (STH) infections is limited by day-to-day variation in helminth egg excretion, confusion with other parasites and the laboratory technicians’ experience. We aimed to estimate the sensitivity and specificity of the Kato-Katz technique to detect infection with Ascaris lumbricoides, hookworm and Trichuris trichiura using a Bayesian approach in the absence of a ‘gold standard’. Data were obtained from a longitudinal study conducted between January 2004 and December 2005 in Samar Province, the Philippines. Each participant provided between one and three stool samples over consecutive days. Stool samples were examined using the Kato-Katz technique and reported as positive or negative for STHs. In the presence of measurement error, the true status of each individual is considered as latent data. Using a Bayesian method, we calculated marginal posterior densities of sensitivity and specificity parameters from the product of the likelihood function of observed and latent data. A uniform prior distribution was used (beta distribution: α=1, β=1). A total of 5,624 individuals provided at least one stool sample. One, two and three stool samples were provided by 1,582, 1,893 and 2,149 individuals, respectively. All STHs showed variation in test results from day to day. Sensitivity estimates of the Kato-Katz technique for one stool sample were 96.9% (95% Bayesian Credible Interval [BCI]: 96.1%, 97.6%), 65.2% (60.0%, 69.8%) and 91.4% (90.5%, 92.3%), for A. lumbricoides, hookworm and T. trichiura, respectively. Specificity estimates for one stool sample were 96.1% (95.5%, 96.7%), 93.8% (92.4%, 95.4%) and 94.4% (93.2%, 95.5%), for A. lumbricoides, hookworm and T. trichiura, respectively. Our results show that the Kato-Katz technique can perform with reasonable accuracy with one day’s stool collection for A. lumbricoides and T. trichiura. Low sensitivity of the Kato-Katz for detection of hookworm infection may be related to rapid degeneration of delicate hookworm eggs with time.
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DOI：
10.1016/j.trstmh.2006.04.008
发表时间：
2007-02-01
影响因子：
2.2
作者：
Dacombe, R. J.;Crampin, A. C.;Fine, P. E. M.
通讯作者：
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影响因子：
1.9
作者：
Dendukuri, N;Joseph, L
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影响因子：
2
作者：
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通讯作者：
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影响因子：
1.9
作者：
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通讯作者：
Joseph, L
DOI：
10.1016/0035-9203(81)90148-6
发表时间：
1981-01-01
影响因子：
2.2
作者：
HALL, A
通讯作者：
HALL, A