Algae sense exact temperatures: small heat shock proteins are expressed at the survival threshold temperature in Cyanidioschyzon merolae and Chlamydomonas reinhardtii.
Algae sense exact temperatures: small heat shock proteins are expressed at the survival threshold temperature in Cyanidioschyzon merolae and Chlamydomonas reinhardtii.
复制标题
DOI：
10.1093/gbe/evu216
复制
发表时间：
2014-09-29
影响因子：
3.3
通讯作者：
Misumi O
中科院分区：
文献类型：
--
作者：
Kobayashi Y;Harada N;Nishimura Y;Saito T;Nakamura M;Fujiwara T;Kuroiwa T;Misumi O
原始的红色的Cyanidioschyzon merolae栖息在酸性温泉中，对高达63 °C的热休克处理表现出强大的抵抗力。微阵列分析，以确定该生物体的高温耐受性的关键基因。在已确定的上调基因中，我们重点关注了属于一类独特HSP家族的两种小热休克蛋白（sHSP）。这两个基因在同一条染色体上以反向重复方向并排定位，并共享一个启动子。这两个基因在热休克处理中同时快速上调（比对照多1,000倍）。有趣的是，上调似乎不是由温度差异触发的，而是由绝对温度触发的。在绿色莱茵衣藻中也发现了类似的sHSP结构基因，但莱茵衣藻中这些sHSP编码基因表达的温度阈值与Cy中sHSP基因表达的温度阈值不同。merolae。这些结果表明绝对温度传感系统在单细胞微藻进化和耐高温条件中可能具有重要意义。
The primitive red alga Cyanidioschyzon merolae inhabits acidic hot springs and shows robust resistance to heat shock treatments up to 63 °C. Microarray analysis was performed to identify the key genes underlying the high temperature tolerance of this organism. Among the upregulated genes that were identified, we focused on two small heat shock proteins (sHSPs) that belong to a unique class of HSP families. These two genes are located side by side in an inverted repeat orientation on the same chromosome and share a promoter. These two genes were simultaneously and rapidly upregulated in response to heat shock treatment (>1,000-fold more than the control). Interestingly, upregulation appeared to be triggered not by a difference in temperatures, but rather by the absolute temperature. Similar sHSP structural genes have been reported in the green alga Chlamydomonas reinhardtii, but the threshold temperature for the expression of these sHSP-encoding genes in Ch. reinhardtii was different from the threshold temperature for the expression of the sHSP genes from Cy. merolae. These results indicate the possible importance of an absolute temperature sensing system in the evolution and tolerance of high-temperature conditions among unicellular microalgae.
登录
查看更多内容
DOI：
10.1016/0005-2728(74)90063-2
发表时间：
1974-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ARNON, DI;MCSWAIN, BD;WADA, K
通讯作者：
WADA, K
DOI：
10.1073/pnas.85.24.9436
发表时间：
1988-12-01
影响因子：
11.1
作者：
INNIS, MA;MYAMBO, KB;BROW, MAD
通讯作者：
BROW, MAD
影响因子：
64.8
作者：
Matsuzaki, M;Misumi, O;Kuroiwa, T
通讯作者：
Kuroiwa, T
影响因子：
4.8
作者：
Mogk, A;Schlieker, C;Bukau, B
通讯作者：
Bukau, B
影响因子：
3.5
作者：
Downs, CA;Heckathorn, SA
通讯作者：
Heckathorn, SA