Stepwise evolution of Salmonella Typhimurium ST313 causing bloodstream infection in Africa.
Stepwise evolution of Salmonella Typhimurium ST313 causing bloodstream infection in Africa.
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在非洲引起血液感染的鼠伤寒沙门氏菌ST313的逐步进化。
DOI：
10.1038/s41564-020-00836-1
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发表时间：
2021-03
影响因子：
28.3
通讯作者：
Hinton JCD
中科院分区：
文献类型：
--
作者：
Pulford CV;Perez-Sepulveda BM;Canals R;Bevington JA;Bengtsson RJ;Wenner N;Rodwell EV;Kumwenda B;Zhu X;Bennett RJ;Stenhouse GE;Malaka De Silva P;Webster HJ;Bengoechea JA;Dumigan A;Tran-Dien A;Prakash R;Banda HC;Alufandika L;Mautanga MP;Bowers-Barnard A;Beliavskaia AY;Predeus AV;Rowe WPM;Darby AC;Hall N;Weill FX;Gordon MA;Feasey NA;Baker KS;Hinton JCD
非伤寒沙门氏菌引起的血液感染是非洲的一个主要公共卫生问题，每年造成约49,600人死亡。与侵袭性非伤寒沙门氏菌病相关的最常见的肠道沙门氏菌致病变种是鼠伤寒沙门氏菌序列类型(ST)313。有人提出，抗菌素耐药性和基因组退化是ST313谱系在非洲取得成功的原因，但这种变化的进化轨迹尚不清楚。在这里，为了定义ST313的进化动力学，我们从1966年至2018年的两个全面的非洲血液感染患者沙门氏菌分离株中进行了亚采样。由此产生的680个基因组序列导致发现了一个泛敏感的ST313谱系(ST313 L3)，该谱系于2016年在马拉维出现，与在英国和巴西导致胃肠道疾病的ST313变体密切相关。基因组分析显示，ST313 L3的重要毒力基因发生了降解，这在其他ST313家系中没有发生。ST313 L3是介于ST313 L1和L2之间的一个系统发育中间体，具有典型的辅助基因组特征。我们深入的遗传型和表型特征确定了在整个非洲入侵鼠伤寒沙门氏菌逐步进化过程中发生的关键功能丧失遗传事件。通过对50年期间收集的分离株的基因和表型分析，确定了非洲入侵的鼠伤寒沙门氏菌的逐步进化。
Bloodstream infections caused by nontyphoidal Salmonella are a major public health concern in Africa, causing ~49,600 deaths every year. The most common Salmonella enterica pathovariant associated with invasive nontyphoidal Salmonella disease is Salmonella Typhimurium sequence type (ST)313. It has been proposed that antimicrobial resistance and genome degradation has contributed to the success of ST313 lineages in Africa, but the evolutionary trajectory of such changes was unclear. Here, to define the evolutionary dynamics of ST313, we sub-sampled from two comprehensive collections of Salmonella isolates from African patients with bloodstream infections, spanning 1966 to 2018. The resulting 680 genome sequences led to the discovery of a pan-susceptible ST313 lineage (ST313 L3), which emerged in Malawi in 2016 and is closely related to ST313 variants that cause gastrointestinal disease in the United Kingdom and Brazil. Genomic analysis revealed degradation events in important virulence genes in ST313 L3, which had not occurred in other ST313 lineages. Despite arising only recently in the clinic, ST313 L3 is a phylogenetic intermediate between ST313 L1 and L2, with a characteristic accessory genome. Our in-depth genotypic and phenotypic characterization identifies the crucial loss-of-function genetic events that occurred during the stepwise evolution of invasive S. Typhimurium across Africa. Stepwise evolution of invasive Salmonella Typhimurium in Africa is defined using genotypic and phenotypic analyses of isolates collected over a 50-yr period.
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影响因子：
168.9
作者：
Feasey, Nicholas A.;Dougan, Gordon;Kingsley, Robert A.;Heyderman, Robert S.;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
11.8
作者：
Feasey, Nicholas A.;Masesa, Clemens;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
3.6
作者：
Figueroa-Bossi, N;Uzzau, S;Bossi, L
通讯作者：
Bossi, L
影响因子：
30.8
作者：
Feasey, Nicholas A.;Hadfield, James;Keddy, Karen H.;Dallman, Timothy J.;Jacobs, Jan;Deng, Xiangyu;Wigley, Paul;Barquist, Lars Barquist;Langridge, Gemma C.;Feltwell, Theresa;Harris, Simon R.;Mather, Alison E.;Fookes, Maria;Aslett, Martin;Msefula, Chisomo;Kariuki, Samuel;Maclennan, Calman A.;Onsare, Robert S.;Weill, Francois-Xavier;Le Hello, Simon;Smith, Anthony M.;McClelland, Michael;Desai, Prerak;Parry, Christopher M.;Cheesbrough, John;French, Neil;Campos, Josefina;Chabalgoity, Jose A.;Betancor, Laura;Hopkins, Katie L.;Nair, Satheesh;Humphrey, Tom J.;Lunguya, Octavie;Cogan, Tristan A.;Tapia, Milagritos D.;Sow, Samba O.;Tennant, Sharon M.;Bornstein, Kristin;Levine, Myron M.;Lacharme-Lora, Lizeth;Everett, Dean B.;Kingsley, Robert A.;Parkhill, Julian;Heyderman, Robert S.;Dougan, Gordon;Gordon, Melita A.;Thomson, Nicholas R.
通讯作者：
Thomson, Nicholas R.
影响因子：
12.3
作者：
Jain M;Olsen HE;Paten B;Akeson M
通讯作者：
Akeson M