Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens.
Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens.
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D10.G4.1 辅助 T 细胞系的亚克隆 (D10S) 的表征，该细胞系在不存在有丝分裂原的情况下增殖至阿托摩尔浓度的白介素-1。
DOI：
10.1016/1043-4666(89)91044-2
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发表时间：
1989
期刊：
影响因子：
3.8
通讯作者：
Dinarello,CA
中科院分区：
文献类型：
--
作者：
Orencole,SF;Dinarello,CA
大量研究表明，白细胞介素-1（IL-1）在T淋巴细胞被有丝分裂原或抗原激活和增殖中起辅助或共刺激作用。我们在此描述了小鼠D10.G4.1辅助性T细胞的稳定亚克隆（D10 S），其在不存在有丝分裂原的情况下增殖至亚飞摩尔（阿托摩尔）浓度的IL-1β或α。D10 S细胞已在培养物中维持超过两年，没有脾细胞饲养层也没有抗原刺激。增殖的检测可以通过在72小时或在48小时内摄取氚化胸苷，通过比色法测量线粒体DNA酶;后一种测定法是快速和廉价的。D10 S细胞不同于亲本克隆D10.G4.，其需要有丝分裂原以获得IL-1活性。IL-1诱导的增殖与IL-2、IL-4或IL-6的产生无关，尽管与IL-1相比，这些细胞以相当高的浓度增殖为这些淋巴因子。D10 S细胞增殖与IL-1在培养物中存在的持续时间直接相关。我们没有发现IL-1在这些细胞中诱导更多IL-1的证据。该亚克隆对IL-1具有高度特异性：未观察到对内毒素、人或鼠干扰素-γ（IFNγ）、肿瘤坏死因子（TNF）、巨噬细胞毒素或粒细胞-巨噬细胞集落刺激因子（GM-CSF）的增殖。转化生长因子β（TGFβ）无抑制作用。仅在高浓度（100 ng/ml）时IL-6才诱导增殖。我们的结论是，这种稳定的、无饲养层的细胞系对IL-1高度敏感，IL-1可作为这些细胞的直接刺激物;它们也可用于生物测定以及IL-1受体的研究，如随附论文所述。
Most studies have shown that interleukin-1 (IL-1) acts as a helper or co-stimulator in T-lymphocyte activation and proliferation by mitogens or antigens. We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1β or α in the absence of mitogens. D10S cells have been maintained in culture for over two years without splenic cell feeder layers nor antigen stimulation. Detection of proliferation can be made by either uptake of tritiated thymidine at 72 h or in 48 h by a colorimetric assay which measures mitochondrial dehydrogenases; the latter assay is rapid and inexpensive. D10S cells are distinct from the parent clone D10.G4., which requires mitogens for IL-1 activity. IL-1-induced proliferation is independent of the elaboration of IL-2, IL-4, or IL-6, although these cells proliferate to these lymphokines at considerably higher concentrations when compared to IL-1. The D10S cells proliferate in direct correlation to the duration of IL-1 presence in the culture. We found no evidence that IL-1 induced more IL-1 in these cells. The subclone is highly specific for IL-1: proliferation was not observed to endotoxin, human or murine interferon-γ (IFNγ), tumor necrosis factor (TNF), lymphotoxin, or granulocyte-macrophage colony stimulating factor (GM-CSF). There was no suppressive effect of transforming growth factor (TGFβ). Only at high concentrations (100 ng/ml) did IL-6 induce proliferation. We conclude that this stable, feeder layer-free cell line is highly sensitive to IL-1 which acts as a direct stimulant for these cells; they are also useful for bioassays as well as the study of IL-1 receptors as described in the accompanying paper.
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影响因子：
4.4
作者：
S. Warner;K. Auger;P. Libby
通讯作者：
S. Warner;K. Auger;P. Libby
影响因子：
64.8
作者：
OHARA, J;PAUL, WE
通讯作者：
PAUL, WE
胸腺瘤细胞白细胞介素 1 依赖性诱导白细胞介素 2 分泌和白细胞介素 2 受体表达。
影响因子：
4.4
作者：
J. Lowenthal;J. Cerottini;H. Macdonald
通讯作者：
H. Macdonald
PMA 和 IL 1 独立且协同的胸腺细胞增殖活性。
影响因子：
4.4
作者：
T. Krakauer;D. Mizel;J. Oppenheim
通讯作者：
J. Oppenheim
T 细胞的自分泌生长不依赖于白细胞介素 2：鉴定白细胞介素 4（IL 4、BSF-1）作为克隆的抗原特异性辅助 T 细胞的自分泌生长因子。
影响因子：
4.4
作者：
T. Kupper;M. Horowitz;F. Lee;R. Robb;P. Flood
通讯作者：
P. Flood