An Integrative Analysis of Transcriptomics and Proteomics Reveals Novel Insights into the Response in the Midgut of Spodoptera frugiperda Larvae to Vip3Aa.
An Integrative Analysis of Transcriptomics and Proteomics Reveals Novel Insights into the Response in the Midgut of Spodoptera frugiperda Larvae to Vip3Aa.
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转录组学和蛋白质组学的综合分析揭示了对Spodoptera frugiperda幼虫对VIP3AA的中肠的反应的新见解。
DOI：
10.3390/toxins14010055
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发表时间：
2022-01-13
期刊：
影响因子：
4.2
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Jin M;Shan Y;Peng Y;Wang P;Li Q;Yu S;Zhang L;Xiao Y
由苏云金芽孢杆菌（Bt）在其营养生长阶段分泌的杀虫Vip3蛋白目前用于Bt作物中以控制昆虫害虫，并且在遗传上不同于已知的杀虫Cry蛋白。与Cry毒素相比，Vip3毒素的机制仍然知之甚少。在这里，草地贪夜蛾幼虫的反应后Vip3Aa的挑战的特点。使用转录组学和蛋白质组学的综合分析，我们发现Vip3Aa对各种途径具有巨大的影响。下调的基因和蛋白主要集中在昆虫激素合成途径等代谢途径，而上调的基因和蛋白主要参与caspase介导的凋亡途径，沿着MAPK信号通路和内吞作用途径。此外，我们还发现了一些重要的候选基因参与细胞凋亡和MAPKs。本研究表明，S。Vip3Aa激活细胞凋亡途径，导致细胞死亡。该结果将促进我们对宿主对Vip3Aa反应过程的理解，并有助于我们更好地了解Vip3A毒素的作用方式。
The insecticidal Vip3 proteins, secreted by Bacillus thuringiensis (Bt) during its vegetative growth phase, are currently used in Bt crops to control insect pests, and are genetically distinct from known insecticidal Cry proteins. Compared with Cry toxins, the mechanisms of Vip3 toxins are still poorly understood. Here, the responses of Spodoptera frugiperda larvae after Vip3Aa challenge are characterized. Using an integrative analysis of transcriptomics and proteomics, we found that Vip3Aa has enormous implications for various pathways. The downregulated genes and proteins were mainly enriched in metabolic pathways, including the insect hormone synthesis pathway, whereas the upregulated genes and proteins were mainly involved in the caspase-mediated apoptosis pathway, along with the MAPK signaling and endocytosis pathways. Moreover, we also identified some important candidate genes involved in apoptosis and MAPKs. The present study shows that exposure of S. frugiperda larvae to Vip3Aa activates apoptosis pathways, leading to cell death. The results will promote our understanding of the host response process to the Vip3Aa, and help us to better understand the mode of action of Vip3A toxins.
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