Proteomic analysis of photosystem I components from different plant species
Proteomic analysis of photosystem I components from different plant species
复制标题
不同植物物种光系统 I 组分的蛋白质组学分析
作者：
L. Zolla;S. Rinalducci;A. Timperio
在这项研究中，通过RP-HPLC分离了类囊体膜基质层中存在的光系统I（PSI）高度疏水蛋白，并通过溶液中胰蛋白酶消化肽片段指纹图谱或通过完整质量测量（IMM）与DNA序列预期值之间的密切对应关系进行鉴定。通过MS/MS进行的蛋白质鉴定与IMM一样可靠。因此，IMM是一种简单有效的方法，用于识别蛋白质没有PTM。本文报道了所有PSI蛋白在10个不同的物种，包括那些尚未被克隆的基因的先生。Lhca 5在四个物种中被明确揭示，证实它确实是属于PSI捕光天线的蛋白质。在所有被研究的物种中，Lhca 6基因的产物从未被发现。关于核心蛋白，Psa-O已在三个物种中发现; Psa-D和Psa-E的同种型已在单子叶植物和双子叶植物中发现。小蛋白质如Psa-I和Psa-J可以很好地分离和鉴定。RP-HPLC产生可靠的指纹图谱，并显示PSI蛋白的相对量似乎明显不同。
In this study, the photosystem I (PSI) highly hydrophobic proteins present within stroma lamellae of the thylakoid membrane were separated by RP‐HPLC and identified either by in‐solution trypsin digestion peptide fragment fingerprinting or by the close correspondence between the intact mass measurements (IMMs) and those expected from the DNA sequence. Protein identification performed by MS/MS was as reliable as IMMs. Thus, IMM is an easy and valid method for identifying proteins that have no PTMs. This paper reports the Mr for all PSI proteins in ten different species, including those whose genes have not yet been cloned. Lhca5 was revealed unequivocally in four species, corroborating that it is indeed a protein belonging to the light‐harvesting antenna of PSI. In all species examined, the product of the Lhca6 gene has never been revealed. Concerning core proteins, Psa‐O has been revealed in three species; isoforms of Psa‐D and Psa‐E have been found in both monocots and dicots. Small proteins like Psa‐I and Psa‐J are well separated and identified. RP‐HPLC produces reliable fingerprints and reveals that the relative amounts of PSI proteins appear to be markedly different.
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影响因子：
2.9
作者：
FLING, SP;GREGERSON, DS
通讯作者：
GREGERSON, DS
蓝藻聚球藻 PsaE 的三维溶液结构。
影响因子：
2.9
作者：
Falzone,CJ;Kao,YH;Zhao,J;Bryant,DA;Lecomte,JT
通讯作者：
Lecomte,JT
DOI：
10.1073/pnas.84.24.8844
发表时间：
1987-12-01
影响因子：
11.1
作者：
HOFFMAN, NE;PICHERSKY, E;CASHMORE, AR
通讯作者：
CASHMORE, AR
PsaE 的 1H 和 15N NMR 归属，PsaE 是蓝藻聚球藻属的光系统 I 亚基。
影响因子：
2.9
作者：
Falzone,CJ;Kao,YH;Zhao,J;MacLaughlin,KL;Bryant,DA;Lecomte,JT
通讯作者：
Lecomte,JT