Noninvasive intratissue refractive index shaping (IRIS) of the cornea with blue femtosecond laser light.
Noninvasive intratissue refractive index shaping (IRIS) of the cornea with blue femtosecond laser light.
复制标题
使用蓝色飞秒激光对角膜进行无创组织内折射率整形 (IRIS)。
DOI：
10.1167/iovs.11-7323
复制
发表时间：
2011
影响因子：
4.4
通讯作者：
Huxlin,KrystelR
中科院分区：
文献类型：
--
作者：
Xu,Lisen;Knox,WayneH;DeMagistris,Margaret;Wang,Nadan;Huxlin,KrystelR
目的。目的：探讨利用400 nm飞秒激光脉冲（blue-IRIS）对活体角膜进行组织内折射率整形的可行性。为了验证角膜固有的双光子吸收使得蓝光虹膜比使用800 nm飞秒激光脉冲更有效的假设。方法：在死后获得新鲜的猫角膜，切成六个楔形。使用波长为400 nm、脉冲持续时间为100 fs、频率为80 MHz的蓝色激光脉冲，以1 ~ 15 mm/s的扫描速度将相位光栅微加工到每个角膜楔中。光栅线宽1 μm，间距5 μm，距角膜前表面150 μm。通过记录内切光栅的衍射效率，立即测量了微加工区域的折射率变化。6小时后，对角膜进行组织学处理，TUNEL染色评估蓝色虹膜是否导致细胞死亡。结果：以1和2 mm/s的速度扫描可引起明显的角膜损伤，表现为气泡和烧伤。在更快的扫描速度下（5、10和15 mm/s），在角膜基质中产生相位光栅，显示出0.037到0.021量级的纯RI变化。RI变化幅度与扫描速度呈负相关。结论：在活体角膜中进行Blue-IRIS是安全有效的。与近红外激光脉冲相比，blue-IRIS提高了可实现的RI变化和扫描速度，而无需在组织中掺杂双光子敏化剂，增加了该技术的临床适用性。
Purpose.: To test the feasibility of intratissue refractive index shaping (IRIS) in living corneas by using 400-nm femtosecond (fs) laser pulses (blue-IRIS). To test the hypothesis that the intrinsic two-photon absorption of the cornea allows blue-IRIS to be performed with greater efficacy than when using 800-nm femtosecond laser pulses.Methods.: Fresh cat corneas were obtained postmortem and cut into six wedges. Blue laser pulses at 400 nm, with 100-fs pulse duration at 80 MHz were used to micromachine phase gratings into each corneal wedge at scanning speeds from 1 to 15 mm/s. Grating lines were 1 μm wide, 5 μm apart, and 150 μm below the anterior corneal surface. Refractive index (RI) changes in micromachined regions were measured immediately by recording the diffraction efficiency of inscribed gratings. Six hours later, the corneas were processed for histology, and TUNEL staining was performed to assess whether blue-IRIS causes cell death.Results.: Scanning at 1 and 2 mm/s caused overt corneal damage in the form of bubbles and burns. At faster scanning speeds (5, 10, and 15 mm/s), phase gratings were created in the corneal stroma, which were shown to be pure RI changes ranging from 0.037 to 0.021 in magnitude. The magnitude of RI change was inversely related to scanning speed. TUNEL staining showed cell death only around bubbles and burns.Conclusions.: Blue-IRIS can be performed safely and effectively in living cornea. Compared with near-infrared laser pulses, blue-IRIS enhances both achievable RI change and scanning speed without the need to dope the tissue with two-photon sensitizers, increasing the clinical applicability of this technique.
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影响因子：
4.4
作者：
S. Wilson;W. J. Kim
通讯作者：
S. Wilson;W. J. Kim
准分子激光原位角膜磨镶术和屈光性角膜切削术矫正高度近视。
DOI：
10.3928/1081-597x-19940901-07
发表时间：
1994
期刊：
Journal of refractive and corneal surgery
影响因子：
--
作者：
I. Pallikaris;D. Siganos
通讯作者：
D. Siganos
影响因子：
2.8
作者：
N. Yokoi;S. Kinoshita
通讯作者：
N. Yokoi;S. Kinoshita
影响因子：
4.4
作者：
Holzer, Mike P.;Rabsilber, Tanja M.;Auffarth, Gerd U.
通讯作者：
Auffarth, Gerd U.
影响因子：
3.7
作者：
Stonecipher, Karl;Ignacio, Teresa S.;Stonecipher, Megan
通讯作者：
Stonecipher, Megan