Impaired CXCR4 expression and cell engraftment of bone marrow-derived cells from aged atherogenic mice.
Impaired CXCR4 expression and cell engraftment of bone marrow-derived cells from aged atherogenic mice.
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DOI：
10.1016/j.atherosclerosis.2011.07.118
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发表时间：
2011-11
期刊：
影响因子：
5.3
通讯作者：
Yu, Hong
中科院分区：
文献类型：
--
作者：
Xu, Qiyuan;Wang, Jian'An;He, Jinlin;Zhou, Mingsheng;Adi, Jennipher;Webster, Keith A.;Yu, Hong
循环祖细胞数量和活性的减少与衰老有关，并与冠状动脉疾病有关。为了确定衰老和动脉粥样硬化疾病对骨髓源性细胞（bmmc）趋化活性的影响，我们检测了衰老和动脉粥样硬化小鼠骨髓源性细胞中CXCR4表面的表达。研究人员比较了年轻（6周龄）ApoE缺失小鼠（ApoE−/−）和喂养6个月高脂肪、高胆固醇饮食的老年ApoE−/−小鼠bmc中CXCR4的表达和细胞移动性。年龄和动脉粥样硬化与CXCR4表面表达显著降低相关，且钙的诱导作用较弱。受损的钙反应与有缺陷的钙内流有关，并通过钙离子载体离子霉素治疗部分恢复。高脂饲料喂养6个月的ApoE−/−小鼠CXCR4表达和SDF-1调控缺陷与24个月大的野生型小鼠相当。老年、致动脉粥样硬化的ApoE−/−小鼠的bmc也显示出SDF-1的归巢缺陷，并且动物的血清和骨髓中SDF-1水平较低。ApoE−/−小鼠动脉粥样硬化的演变与BMCs移动性的逐渐丧失、CXCR4表达的降低以及血清和骨髓中SDF-1水平的降低是平行的。这些变化抑制了bmc的归巢能力，并可能导致该模型中动脉粥样硬化的进展。
Reduced numbers and activity of circulating progenitor cells are associated with aging and have been linked with coronary artery disease. To determine the impact of aging and atherosclerotic disease on the chemotaxic activity of bone marrow derived cells (BMCs), we examined CXCR4 surface expression on BMCs from aged and atherosclerotic mice. CXCR4 expression and cellular mobility were compared between BMCs of young (6-week old) ApoE null mice (ApoE−/−) and aged ApoE−/− mice that had been fed with a high-fat, high-cholesterol diet for 6-months. Age and atherosclerosis correlated with significantly lower surface expression of CXCR4 that was less inducible by calcium. The impaired calcium response was associated with defective calcium influx and was partially recovered by treatment with the calcium ionophore ionomycin. ApoE−/− mice fed high fat diet for 6-months had defective CXCR4 expression and SDF-1 regulation that is equivalent to that of 24-month old wild type mice. BMCs from aged, atherogenic ApoE−/− mice also displayed defective homing to SDF-1, and the animals had lower serum and bone marrow levels of SDF-1. Evolution of atherosclerosis in ApoE−/− mice is paralleled by progressive loss of mobility of BMCs with reductions of CXCR4 expression, and reduced levels of SDF-1 in both serum and bone marrow. These changes mute the homing capability of BMCs and may contribute to the progression of atherosclerosis in this model.
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影响因子：
24
作者：
Heiss, C;Keymel, S;Kalka, C
通讯作者：
Kalka, C
影响因子：
37.8
作者：
Yoon, CH;Hur, J;Kim, HS
通讯作者：
Kim, HS
影响因子：
20.1
作者：
Vasa, M;Fichtlscherer, S;Dimmeler, S
通讯作者：
Dimmeler, S
DOI：
10.3324/haematol.13206
发表时间：
2009-02-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
Foguenne, Jacques;Di Stefano, Ivano;Gothot, Andre
通讯作者：
Gothot, Andre
影响因子：
20.1
作者：
Wassmann, Sven;Werner, Nikos;Nickenig, Georg
通讯作者：
Nickenig, Georg