A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control.
A plant-specific transcription factor IIB-related protein, pBRP2, is involved in endosperm growth control.
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DOI：
10.1371/journal.pone.0017216
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发表时间：
2011-02-24
期刊：
影响因子：
3.7
通讯作者：
Lagrange T
中科院分区：
文献类型：
--
作者：
Cavel E;Pillot M;Pontier D;Lahmy S;Bies-Etheve N;Vega D;Grimanelli D;Lagrange T
一般转录因子IIB（TFIIB）和TFIIB相关因子（BRF）是RNA聚合酶II/III（RNAPII/III）选择性的保守因子，它们参与真核生物中聚合酶的募集和转录起始。最近的研究结果表明，植物已经进化出第三种类型的B因子，植物特异性TFIIB相关蛋白1（pBRP 1），它似乎参与RNAPI转录。在这里，我们通过报告一种新的TFIIB相关蛋白，植物特异性TFIIB相关蛋白2（pBRP 2），这是迄今为止发现的只有在拟南芥家族的表征，扩展了B因子在植物中的库。与其他B因子普遍表达不同，PBRP 2的表达仅限于生殖器官和种子，如RT-PCR、免疫荧光标记和GUS染色实验所示。有趣的是，pbrp 2功能丧失特异性地影响合胞胚乳的发育，野生型发育需要双亲的贡献。pBRP 2是第一个在真核生物中表现出细胞特异性表达和调节的B因子，并且可能在被子植物中执行双亲繁殖中发挥作用。
General transcription factor IIB (TFIIB) and TFIIB-related factor (BRF), are conserved RNA polymerase II/III (RNAPII/III) selectivity factors that are involved in polymerase recruitment and transcription initiation in eukaryotes. Recent findings have shown that plants have evolved a third type of B-factor, plant-specific TFIIB-related protein 1 (pBRP1), which seems to be involved in RNAPI transcription. Here, we extend the repertoire of B-factors in plants by reporting the characterization of a novel TFIIB-related protein, plant-specific TFIIB-related protein 2 (pBRP2), which is found to date only in the Brassicacea family. Unlike other B-factors that are ubiquitously expressed, PBRP2 expression is restricted to reproductive organs and seeds as shown by RT-PCR, immunofluorescence labelling and GUS staining experiments. Interestingly, pbrp2 loss-of-function specifically affects the development of the syncytial endosperm, with both parental contributions required for wild-type development. pBRP2, is the first B-factor to exhibit cell-specific expression and regulation in eukaryotes, and might play a role in enforcing bi-parental reproduction in angiosperms.
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