Pluripotent stem cell heterogeneity and the evolving role of proteomic technologies in stem cell biology.
Pluripotent stem cell heterogeneity and the evolving role of proteomic technologies in stem cell biology.
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DOI：
10.1002/pmic.201100100
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发表时间：
2011-10
期刊：
影响因子：
3.4
通讯作者：
Boheler, Kenneth R.
中科院分区：
文献类型：
--
作者：
Gundry, Rebekah L.;Burridge, Paul W.;Boheler, Kenneth R.
干细胞代表了再生医学的明显选择，对人类发育和药物测试的研究是无价的。特别是多能干细胞（PSC）的蛋白质组学景观尚未明确定义;因此，这一研究领域将大大受益于旨在更好地表征这些细胞的协同努力。在这篇简明的综述中，我们概述了干细胞的潜能，强调了PSC异质性的类型和实际意义，并详细分析了多能蛋白质组在这一快速发展领域的独特资源中的当前观点。我们在这篇综述中的目标是提供具体的见解，目前的状态，已知的蛋白质组的小鼠和人类PSC。这已经通过将已发表的数据整合到统一的PSC蛋白质组中来实现，以促进蛋白质的鉴定，这可能为干细胞状态提供信息，并揭示我们目前观点有限的领域。这些分析提供了对PSC蛋白质组学分析所面临的挑战的深入了解，并揭示了一个领域-细胞表面亚蛋白质组-这将特别受益于加强研究工作。
Stem cells represent obvious choices for regenerative medicine and are invaluable for studies of human development and drug testing. The proteomic landscape of pluripotent stem cells (PSCs), in particular, is not yet clearly defined; consequently, this field of research would greatly benefit from concerted efforts designed to better characterize these cells. In this concise review, we provide an overview of stem cell potency, highlight the types and practical implications of heterogeneity in PSCs and provide a detailed analysis of the current view of the pluripotent proteome in a unique resource for this rapidly evolving field. Our goal in this review is to provide specific insights into the current status of the known proteome of both mouse and human PSCs. This has been accomplished by integrating published data into a unified PSC proteome to facilitate the identification of proteins, which may be informative for the stem cell state as well as to reveal areas where our current view is limited. These analyses provide insight into the challenges faced in the proteomic analysis of PSCs and reveal one area – the cell surface subproteome – that would especially benefit from enhanced research efforts.
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影响因子：
6.7
作者：
Boheler KR
通讯作者：
Boheler KR
影响因子：
23.9
作者：
Fuchs E
通讯作者：
Fuchs E
影响因子：
4.4
作者：
Dormeyer, Wilma;van Hoof, Dennis;Krijgsveld, Jeroen
通讯作者：
Krijgsveld, Jeroen
DOI：
10.1016/j.bbrc.2006.08.151
发表时间：
2006-10-27
影响因子：
3.1
作者：
Baharvand, Hossein;Hajheidari, Mohsen;Salekdeh, Ghasem Hosseini
通讯作者：
Salekdeh, Ghasem Hosseini
影响因子：
3.4
作者：
Chaerkady, Raghothama;Kerr, Candace L.;Kandasamy, Kumaran;Marimuthu, Arivusudar;Gearhart, John D.;Pandey, Akhilesh
通讯作者：
Pandey, Akhilesh