AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana

AtARRE, an E3 ubiquitin ligase, negatively regulates ABA signaling in Arabidopsis thaliana
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AtARRE 是一种 E3 泛素连接酶,可负向调节拟南芥中的 ABA 信号传导

DOI:
10.1007/s00299-018-2311-8
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发表时间:
2018-06
期刊:
影响因子:
6.2
通讯作者:
Yang Yi
Yang Yi
中科院分区:
生物学2区
文献类型:
--
作者:
Wang Boya;Li Chuzhe;Kong Xiangge;Li Ying;Liu Zhibin;Wang Jianmei;Li Xufeng;Yang Yi

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RING 型 E3 连接酶 AtARRE 作为负调节因子参与植物 ABA 响应。泛素化蛋白酶系统(UPS)在转录后调控中具有重要意义。在 UPS 中,E3 连接酶识别底物蛋白并将多聚泛素链介导到底物上。在这里,我们鉴定了一个新基因,命名为拟南芥ABA相关环型E3连接酶(AtARRE),该基因由ABA和NaCl诱导。 AtARRE 编码位于拟南芥细胞核和质膜中的功能性 RING 型 E3 连接酶蛋白。生理分析表明,AtARRE(T-DNA 插入突变体 atarre-1 和 atarre-2)的突变导致植物对 ABA 过敏,包括增强气孔闭合、减少根伸长和种子萌发。然而,与WT和突变atarre植物相比,AtARRE转基因株系的过度表达导致植物对ABA不敏感。 ABA处理下,atarre突变体植株中ABA响应基因RD29A、RD29B、RD22和ABI5的转录本丰度显着高于WT和AtARRE过表达株系。因此,这些结果表明 AtARRE 在拟南芥中充当 ABA 介导的应激反应的负调节因子。
The RING-type E3 ligase AtARRE participates in the plant ABA responding as a negative regulator. Ubiquitination protease system (UPS) is significant in post-transcriptional regulation. In UPS, E3 ligase recognizes the substrate protein and mediates the polyubiquitin chain onto the substrate. Here, we identified a new gene, named Arabidopsis thaliana ABA-related RING-type E3 ligase (AtARRE), which induced by ABA and NaCl. AtARRE encodes a functional RING-type E3 ligase protein localized in nucleus and plasma membrane of Arabidopsis. Physiological analysis demonstrated that mutation of AtARRE (T-DNA insert mutants atarre-1 and atarre-2) caused plants hypersensitivity to ABA, including enhanced stomatal closure, reduced root elongation and seed germination. However, overexpression of AtARRE transgenic lines caused plants hyposensitive to ABA compared with WT and mutant atarre plants. Under the treatment of ABA, the transcript abundances of ABA-responsive genes RD29A, RD29B, RD22 and ABI5 in atarre mutant plants were markedly higher than those of WT and AtARRE overexpression lines. Hence, these results indicate that AtARRE acts as a negative regulator of ABA-mediated stress responses in Arabidopsis.
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