Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb
Multiple High-Affinity K+ Transporters and ABC Transporters Involved in K+ Uptake/Transport in the Potassium-Hyperaccumulator Plant Phytolacca acinosa Roxb
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多个高亲和力 K 转运蛋白和 ABC 转运蛋白参与钾超积累植物商陆 Roxb 的 K 吸收/转运
DOI：
10.3390/plants9040470
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发表时间：
2020-04
期刊：
影响因子：
--
通讯作者：
Yi Su
中科院分区：
文献类型：
--
作者：
Qin Xie;Liying Ma;Peng Tan;Deng Wentao;Chao Huang;Deming Liu;Wanhuang Lin;Yi Su
钾是植物生长发育的重要必需元素。长期缺钾可导致植物严重缺钾表型。有趣的是，商陆是一种钾含量异常高的植物，即使在严重缺钾的土壤中也能生长良好并完成其生命周期。结果表明，茎、叶是其高钾积累的主要组织，且根对K+的吸收能力强，地上部对K+的积累能力强。对植株生长和生理特性的分析表明，商陆对外源钾的适应性较强。为揭示钾超积累植物商陆K+吸收和转运的机制，采用转录组测序技术筛选K+吸收/转运相关基因，并比较其在K+饥饿植株和正常培养植株中的表达谱。结果表明，在刺叶侧耳中鉴定出18个HAK/KT/KUPs成员、10个AKT成员和1个HKT成员。其中6个HAKs、2个AKT和PaHKT 1的表达差异有统计学意义。这些转运蛋白可能协同参与了商陆对K+的吸收/转运，从而促进了商陆对K+的高积累。此外，部分ABC转运蛋白表达量的显著变化表明，ABC转运蛋白可能在低K+浓度下对商陆K+的吸收和转运起重要作用。
Potassium is an important essential element for plant growth and development. Long-term potassium deprivation can lead to a severe deficiency phenotype in plants. Interestingly, Phytolacca acinosa is a plant with an unusually high potassium content and can grow well and complete its lifecycle even in severely potassium deficient soil. In this study, we found that its stems and leaves were the main tissues for high potassium accumulation, and P. acinosa showed a strong ability of K+ absorption in roots and a large capability of potassium accumulation in shoots. Analysis of plant growth and physiological characteristics indicated that P. acinosa had an adaptability in a wide range of external potassium levels. To reveal the mechanism of K+ uptake and transport in the potassium-hyperaccumulator plant P. acinosa, K+ uptake-/transport-related genes were screened by transcriptome sequencing, and their expression profiles were compared between K+ starved plants and normal cultured plants. Eighteen members of HAK/KT/KUPs, ten members of AKTs, and one member of HKT were identified in P. acinosa. Among them, six HAKs, and two AKTs and PaHKT1 showed significantly different expression. These transporters might be coordinatively involved in K+ uptake/transport in P. acinosa and lead to high potassium accumulation in plant tissues. In addition, significantly changed expression of some ABC transporters indicated that ABC transporters might be important for K+ uptake and transport in P. acinosa under low K+ concentrations.
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影响因子：
--
作者：
Jiang Chao-qian
通讯作者：
Jiang Chao-qian
DOI：
10.1126/science.1246489
发表时间：
2014-03-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lee JY;Yang JG;Zhitnitsky D;Lewinson O;Rees DC
通讯作者：
Rees DC
影响因子：
7.4
作者：
Buschmann, PH;Vaidyanathan, R;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
7.4
作者：
Bañuelos, MA;Garciadeblas, B;Rodríguez-Navarro, A
通讯作者：
Rodríguez-Navarro, A
DOI：
10.1073/pnas.1009777108
发表时间：
2011-01-11
影响因子：
11.1
作者：
Gajdanowicz, Pawel;Michard, Erwan;Dreyer, Ingo
通讯作者：
Dreyer, Ingo