Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas.
Gene silencing of Sugar-dependent 1 (JcSDP1), encoding a patatin-domain triacylglycerol lipase, enhances seed oil accumulation in Jatropha curcas.
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DOI：
10.1186/1754-6834-7-36
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发表时间：
2014-03-08
影响因子：
6.3
通讯作者：
Chua NH
中科院分区：
文献类型：
--
作者：
Kim MJ;Yang SW;Mao HZ;Veena SP;Yin JL;Chua NH
三酰基甘油（TAG）是植物中最丰富的贮藏油形式。它们由三个脂肪酸链（通常为C16或C18）共价连接到甘油。SDP 1是拟南芥种子中TAG催化第一步的特异性脂肪酶。缺乏SDP 1的拟南芥突变体积累高水平的油，可能是由于TAG降解的阻断。我们应用这些知识，从模式植物，拟南芥，工程增加种子油含量的生物柴油植物小桐子使用RNA干扰（RNAi）技术。由于麻风树是一种生物柴油作物，其种子油含量的任何显着增加都将是一个重要的农艺性状。使用.以拟南芥为模式植物，我们发现与野生型（哥伦比亚-0; Col-0）相比，SDP 1的缺乏导致种子中较高的TAG积累和较多数量的油体。我们克隆了麻风树JcSDP 1，并通过拟南芥sdp 1 -5突变体的互补验证了其功能。利用拟南芥的观察，我们使用RNAi技术在转基因麻风树中产生JcSDP 1缺陷。我们发现，与对照（CK; 35 S：GFP）植物相比，麻风树JcSDP 1-RNAi植物积累了高13至30%的总种子储存脂质，沿着7%的蛋白质含量的补偿性降低。种子中的游离脂肪酸（FFA）含量从对照植物的27%降低到JcSDP 1-RNAi植物的8.5%。在这里，我们表明，SDP 1缺乏增强拟南芥种子油的积累。基于这一结果，我们通过RNAi技术用天然JcSDP 1启动子沉默内源JcSDP 1表达来产生SDP 1缺陷型转基因麻风树植物。与对照（CK; 35 S：GFP）植物相比，麻风树JcSDP 1-RNAi植物的种子积累了高达30%的高总脂质，并且具有降低的FFA含量。我们的策略，改善一个重要的农艺性状的麻风树可以扩展到其他油料作物，以产生更高的种子油。
Triacylglycerols (TAGs) are the most abundant form of storage oil in plants. They consist of three fatty acid chains (usually C16 or C18) covalently linked to glycerol. SDP1 is a specific lipase for the first step of TAG catabolism in Arabidopsis seeds. Arabidopsis mutants deficient in SDP1 accumulate high levels of oils, probably due to blockage in TAG degradation. We applied this knowledge from the model plant, Arabidopsis thaliana, to engineer increased seed oil content in the biodiesel plant Jatropha curcas using RNA interference (RNAi) technology. As Jatropha is a biodiesel crop, any significant increase in its seed oil content would be an important agronomic trait. Using A. thaliana as a model plant, we found that a deficiency of SDP1 led to higher TAG accumulation and a larger number of oil bodies in seeds compared with wild type (Columbia-0; Col-0). We cloned Jatropha JcSDP1, and verified its function by complementation of the Arabidopsis sdp1-5 mutant. Taking advantage of the observation with Arabidopsis, we used RNAi technology to generate JcSDP1 deficiency in transgenic Jatropha. We found that Jatropha JcSDP1-RNAi plants accumulated 13 to 30% higher total seed storage lipid, along with a 7% compensatory decrease in protein content, compared with control (CK; 35S:GFP) plants. Free fatty acid (FFA) content in seeds was reduced from 27% in control plants to 8.5% in JcSDP1-RNAi plants. Here, we showed that SDP1 deficiency enhances seed oil accumulation in Arabidopsis. Based on this result, we generated SDP1-deficient transgenic Jatropha plants using by RNAi technology with a native JcSDP1 promoter to silence endogenous JcSDP1 expression. Seeds of Jatropha JcSDP1-RNAi plants accumulated up to 30% higher total lipid and had reduced FFA content compared with control (CK; 35S:GFP) plants. Our strategy of improving an important agronomic trait of Jatropha can be extended to other oil crops to yield higher seed oil.
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影响因子：
4.8
作者：
Eastmond, PJ
通讯作者：
Eastmond, PJ
影响因子：
6.9
作者：
Chia, TYP;Pike, MJ;Rawsthorne, S
通讯作者：
Rawsthorne, S
影响因子：
7.4
作者：
Kelly, Amelie A.;Quettier, Anne-Laure;Eastmond, Peter J.
通讯作者：
Eastmond, Peter J.
影响因子：
4.8
作者：
Kurat, CF;Natter, K;Kohlwein, SD
通讯作者：
Kohlwein, SD
DOI：
10.1073/pnas.1003530107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Le, Brandon H.;Cheng, Chen;Goldberg, Robert B.
通讯作者：
Goldberg, Robert B.