The ability of Group A streptococcus to adhere to immortalized human skin versus throat cell lines does not reflect their predicted tissue tropism.
The ability of Group A streptococcus to adhere to immortalized human skin versus throat cell lines does not reflect their predicted tissue tropism.
复制标题
A 组链球菌相对于咽喉细胞系粘附于永生化人类皮肤的能力并不反映其预测的组织向性。
DOI：
10.1016/j.cmi.2017.03.011
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
T. Proft
中科院分区：
文献类型：
--
作者：
J. Loh;J. C. Tsai;T. Proft
目的探讨A组链球菌对永生化人角质形成细胞系HaCat和咽部细胞系的黏附能力是否反映了A组链球菌的组织趋向性。方法将A组链球菌单层接种人上皮细胞系，计算培养1h后细菌的贴壁率。结果21/69株推测为皮肤嗜性，25/69株为嗜咽性。然而，23/69被归类为多面手的菌株显示出总体上更强的对两种细胞系的黏附能力。结论基于基因模式的预测的组织趋向性并不能通过对特定细胞系的优先黏附来反映，这表明早期黏附事件在确定特定生态位的感染方面可能并不像最初预期的那样重要。
ObjectivesTo determine ifemmpattern-inferred tissue tropisms of Group A streptococcus strains is reflected in their ability to adhere to immortalized human HaCat (keratinocyte) and Detroit 562 (pharyngeal) cell lines.MethodsHuman epithelial cell line monolayers were inoculated with Group A streptococcus, and the percentage of adhered bacteria after a 1-h incubation period was calculated.ResultsOf the 21/69 inferred-skin-tropic, and the 25/69 inferred-throat-tropic isolates no preferential adherence was observed to a particular cell line. The 23/69 strains classified as ‘generalists’, however, showed an overall greater ability to adhere to both cell lines.ConclusionsPredicted tissue-tropism based onemmpatterns is not reflected by preferential adherence to a specific cell line, suggesting that early adhesion events may not be as important in establishing infection at a particular ecological niche than originally expected.
影响因子：
6.4
作者：
Bessen, DE;Sotir, CM;Hollingshead, SK
通讯作者：
Hollingshead, SK