Landscape of the lipidome and transcriptome under heat stress in Arabidopsis thaliana.
Landscape of the lipidome and transcriptome under heat stress in Arabidopsis thaliana.
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DOI：
10.1038/srep10533
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发表时间：
2015-05-27
影响因子：
4.6
通讯作者：
Saito K
中科院分区：
文献类型：
--
作者：
Higashi Y;Okazaki Y;Myouga F;Shinozaki K;Saito K
环境胁迫导致植物膜损伤。脂质研究是了解植物对气候变化适应的必要条件。本研究通过lc - ms脂质组学和基因芯片转录组学分析，阐明了短期热胁迫对拟南芥叶片膜的影响。营养植物在高温下生长一天，然后在正常条件下生长。利用Spearman相关系数对66种检测到的甘油类进行了分类。三酰基甘油、36:4-和36:5-单半乳糖二酰基甘油、34:2-和36:2-双半乳糖二酰基甘油、34:1-、36:1-和36:6-磷脂酰胆碱和34:1-磷脂酰乙醇胺在应激下增加，在恢复过程中立即下降。热胁迫下含有α-亚麻酸（18:3）的一种三酰基甘油（54:9）的相对含量增加。这些结果表明，热胁迫诱导拟南芥叶片中脂质转化的中间体甘油三酯水平升高，并导致膜多不饱和脂肪酸减少。微阵列数据揭示了负责观察到的代谢变化的候选基因。
Environmental stress causes membrane damage in plants. Lipid studies are required to understand the adaptation of plants to climate change. Here, LC-MS-based lipidomic and microarray transcriptome analyses were carried out to elucidate the effect of short-term heat stress on the Arabidopsis thaliana leaf membrane. Vegetative plants were subjected to high temperatures for one day, and then grown under normal conditions. Sixty-six detected glycerolipid species were classified according to patterns of compositional change by Spearman’s correlation coefficient. Triacylglycerols, 36:4- and 36:5-monogalactosyldiacylglycerol, 34:2- and 36:2-digalactosyldiacylglycerol, 34:1-, 36:1- and 36:6-phosphatidylcholine, and 34:1-phosphatidylethanolamine increased by the stress and immediately decreased during recovery. The relative amount of one triacylglycerol species (54:9) containing α-linolenic acid (18:3) increased under heat stress. These results suggest that heat stress in Arabidopsis leaves induces an increase in triacylglycerol levels, which functions as an intermediate of lipid turnover, and results in a decrease in membrane polyunsaturated fatty acids. Microarray data revealed candidate genes responsible for the observed metabolic changes.
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影响因子：
3.6
作者：
Okazaki, Yozo;Kamide, Yukiko;Hirai, Masami Yokota;Saito, Kazuki
通讯作者：
Saito, Kazuki
影响因子：
7.4
作者：
Kaup, MT;Froese, CD;Thompson, JE
通讯作者：
Thompson, JE
DOI：
10.1073/pnas.1318511111
发表时间：
2014-01-21
影响因子：
11.1
作者：
Bates, Philip D.;Johnson, Sean R.;Browse, John
通讯作者：
Browse, John
影响因子：
3.8
作者：
Devaiah, Shivakumar Pattada;Roth, Mary R.;Wang, Xuemin
通讯作者：
Wang, Xuemin
影响因子：
7.4
作者：
KUNST, L;BROWSE, J;SOMERVILLE, C
通讯作者：
SOMERVILLE, C