Chemical probes for analysis of carbonylated proteins: a review.
Chemical probes for analysis of carbonylated proteins: a review.
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DOI：
10.1016/j.jchromb.2010.08.004
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发表时间：
2011-05-15
影响因子：
3
通讯作者：
Forster, Michael J.
中科院分区：
文献类型：
--
作者：
Yan, Liang-Jun;Forster, Michael J.
关键词：
蛋白质羰基化是蛋白质氧化的一种主要形式，被广泛用作氧化应激的指标。羰基不具有区分紫外或可见光、分光光度吸收/荧光特性，因此它们的检测和定量只能使用特定的化学探针来实现。在本文中，我们回顾了几种化学探针的优点和缺点，已经和仍在用于蛋白质羰基分析。这些探针包括2,4 -二硝基苯基肼（DNPH）、氚化硼氢化钠（[3H]NaBH4）、含生物素探针和荧光探针。由于我们的讨论倾向于基于凝胶的方法，因此在适用的情况下说明了这些探针在基于凝胶的二维羰基化蛋白质组学分析中的应用。通过ELISA，免疫荧光成像，近红外荧光检测和无凝胶蛋白质组学方法分析羰基化蛋白也在适当的地方进行了讨论。此外，还讨论了蓝色天然凝胶电泳作为一种工具在二维凝胶基羰基蛋白分析中的潜在应用。
Protein carbonylation is a major form of protein oxidation and is widely used as an indicator of oxidative stress. Carbonyl groups do not have distinguishing UV or visible, spectrophotometric absorbance/fluorescence characteristics and thus their detection and quantification can only be achieved using specific chemical probes. In this paper, we review the advantages and disadvantages of several chemical probes that have been and are still being used for protein carbonyl analysis. These probes include 2, 4-dinitrophenylhydazine (DNPH), tritiated sodium borohydride ([3H]NaBH4), biotin-containing probes, and fluorescence probes. As our discussions lean toward gel-based approaches, utilizations of these probes in 2D gel-based proteomic analysis of carbonylated proteins are illustrated where applicable. Analysis of carbonylated proteins by ELISA, immunofluorescent imaging, near infrared fluorescence detection, and gel-free proteomic approaches are also discussed where appropriate. Additionally, potential applications of blue native gel electrophoresis as a tool for first dimensional separation in 2D gel-based analysis of carbonylated proteins are discussed as well.
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DOI：
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发表时间：
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