Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii.
Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii.
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DOI：
10.1186/1471-2164-15-1020
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发表时间：
2014-11-25
期刊：
影响因子：
4.4
通讯作者：
Brown MH
中科院分区：
文献类型：
--
作者：
Eijkelkamp BA;Stroeher UH;Hassan KA;Paulsen IT;Brown MH
鲍曼不动杆菌是一种重要的医院病原菌，尤其是由于高度耐药菌株的传播。基因组数据显示，鲍曼不动杆菌具有高度的遗传多样性，这与表型水平上的主要变异有关。到目前为止，比较基因组研究的目标是确定鲍曼不动杆菌的抗性决定因素。在这项研究中，我们对这些分析进行了扩展和扩展，以更好地了解八株鲍曼不动杆菌的毒力因素，这些菌株在克隆、时间和地理上是不同的，其中包括一株被认为非致病的菌株和一株社区获得的鲍曼不动杆菌。我们已经在鲍曼不动杆菌基因组中发现了大量已知在其他病原体的毒力中起作用的基因，例如最近研究的VI型分泌系统的Pro-丙氨酸-丙氨酸-精氨酸(PAAR)-重复结构域。不足为奇的是，许多毒力候选者似乎是鲍曼不动杆菌毒力分离物核心基因组的一部分，但在鲍曼不动杆菌无毒菌株SDF中经常被发现插入中断。我们的研究还发现，许多已知或推测的毒力决定因素仅限于特定的克隆谱系，这表明这些毒力决定因素可能是这些广泛存在的常见克隆成功的关键。以往的研究表明，鲍曼不动杆菌的高水平内在和适应性耐药使得鲍曼不动杆菌在医院环境中广泛存在。这似乎促进了其毒力特征谱系的扩展，因为通常情况下，与社区获得的分离物相比，本研究中的医院内菌株拥有更多的毒力基因。已知或推测的毒力因子在本研究的8个菌株中都有主要的遗传变异，这表明鲍曼不动杆菌的毒力机制是复杂的和多方面的。总体而言，这些分析增加了我们对鲍曼不动杆菌致病性的了解，并将有助于未来的研究，确定毒力因子在克隆谱系内和/或跨物种的重要性。本文的在线版本(DOI：10.1186/1471-2164-15-1020)包含补充材料，可供授权用户使用。
Acinetobacter baumannii is a significant hospital pathogen, particularly due to the dissemination of highly multidrug resistant isolates. Genome data have revealed that A. baumannii is highly genetically diverse, which correlates with major variations seen at the phenotypic level. Thus far, comparative genomic studies have been aimed at identifying resistance determinants in A. baumannii. In this study, we extend and expand on these analyses to gain greater insight into the virulence factors across eight A. baumannii strains which are clonally, temporally and geographically distinct, and includes an isolate considered non-pathogenic and a community-acquired A. baumannii. We have identified a large number of genes in the A. baumannii genomes that are known to play a role in virulence in other pathogens, such as the recently studied proline-alanine-alanine-arginine (PAAR)-repeat domains of the type VI secretion systems. Not surprising, many virulence candidates appear to be part of the A. baumannii core genome of virulent isolates but were often found to be insertionally disrupted in the avirulent A. baumannii strain SDF. Our study also reveals that many known or putative virulence determinants are restricted to specific clonal lineages, which suggests that these virulence determinants may be crucial for the success of these widespread common clones. It has previously been suggested that the high level of intrinsic and adaptive resistance has enabled the widespread presence of A. baumannii in the hospital environment. This appears to have facilitated the expansion of its repertoire of virulence traits, as in general, the nosocomial strains in this study possess more virulence genes compared to the community-acquired isolate. Major genetic variation in known or putative virulence factors was seen across the eight strains included in this study, suggesting that virulence mechanisms are complex and multifaceted in A. baumannii. Overall, these analyses increase our understanding of A. baumannii pathogenicity and will assist in future studies determining the significance of virulence factors within clonal lineages and/or across the species. The online version of this article (doi:10.1186/1471-2164-15-1020) contains supplementary material, which is available to authorized users.
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