In vivo serial evaluation of superparamagnetic iron-oxide labeled stem cells by off-resonance positive contrast.
In vivo serial evaluation of superparamagnetic iron-oxide labeled stem cells by off-resonance positive contrast.
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DOI：
10.1002/mrm.21816
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发表时间：
2008-12
影响因子：
3.3
通讯作者：
Yang, Phillip C.
中科院分区：
文献类型：
--
作者：
Suzuki, Yoriyasu;Cunningham, Charle H.;Noguchi, Ken-ichiro;Chen, Ian Y.;Weissman, Irving L.;Yeung, Alan C.;Robbins, Robert C.;Yang, Phillip C.
磁共振成像（MRI）正在成为一种追踪氧化铁标记干细胞的诊断方式。本研究调查了旨在产生 1.5 特斯拉正对比度的偏共振 (OR) 脉冲序列是否可以评估体内输送的干细胞的位置、数量和活力。使用转染荧光素酶报告基因（luc-mESC）的小鼠胚胎干细胞，对OR信号进行多模态验证，以确定在细胞移植到小鼠后肢后是否可以确定超顺磁性氧化铁标记的luc-mESC（SPIO-luc-mESC）的植入参数。与 GRE 序列相比，通过 OR 技术实现了 SPIO-luc-mESC 的信号和噪声对比的显着增加。移植后立即观察到 SPIO-luc-mESC 的数量与 OR 信号之间存在显着相关性（R2=0.74，p<0.05）。通过生物发光成像 (BLI) 对移植细胞活力的评估显示，第 16 天时荧光素酶活性显着增加，而 MRI 信号没有显示差异。未观察到细胞活力的 BLI 和 MRI 信号之间存在显着相关性。总之，使用非共振序列，SPIO标记的干细胞在空间和时间上的精确定位和定量是可能的。然而，非共振序列不允许评估细胞活力。
Magnetic resonance imaging (MRI) is emerging as a diagnostic modality to track iron-oxide labeled stem cells. This study investigates whether an off-resonance (OR) pulse sequence designed to generate positive contrast at 1.5-Tesla can assess location, quantity and viability of delivered stem cells in vivo. Using mouse embryonic stem cell transfected with luciferase reporter gene (luc-mESC), multimodality validation of OR signal was conducted to determine whether engraftment parameters of superparamagnetic iron-oxide labeled luc-mESC (SPIO-luc-mESC) could be determined after cell transplantation into the mouse hind limb. A significant increase in signal- and contrast-to-noise of the SPIO-luc-mESC was achieved by OR technique when compared to GRE sequence. A significant correlation between the quantity of SPIO-luc-mESC and OR signal was observed immediately after transplantation (R2=0.74, p<0.05). The assessment of transplanted cell viability by bioluminescence imaging (BLI) showed significant increase of luciferase activities by day 16 while MRI signal showed no difference. No significant correlation between BLI and MRI signals of cell viability was observed. In conclusion, using off-resonance sequence, the precise localization and quantitation of SPIO-labeled stem cells in both space and time were possible. However, off-resonance sequence did not allow evaluation of cell viability.
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影响因子：
37.8
作者：
Cao, F;Lin, S;Wu, JC
通讯作者：
Wu, JC
影响因子：
5.2
作者：
Li, Zongjin;Suzuki, Yoriyasu;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
158.5
作者：
Schaechinger, Volker;Erbs, Sandra;Zeiher, Andreas M.
通讯作者：
Zeiher, Andreas M.
影响因子：
37.8
作者：
Hill, JM;Dick, AJ;Lederman, RJ
通讯作者：
Lederman, RJ
影响因子：
3.3
作者：
Arai, T;Kofidis, T;Yang, PC
通讯作者：
Yang, PC