Pericentrin Is Related to Abnormal β-Cell Insulin Secretion through F-Actin Regulation in Mice.
Pericentrin Is Related to Abnormal β-Cell Insulin Secretion through F-Actin Regulation in Mice.
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小鼠中 Pericentrin 通过 F-肌动蛋白调节与异常 β 细胞胰岛素分泌相关
DOI:
10.1371/journal.pone.0130458
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Chen X
中科院分区:
文献类型:
--
作者:
Zu Y;Gong Y;Wan L;Lv Y;Cui S;Jin X;Li C;Chen X
The aim of this study was to investigate the regulating effect of pericentrin (PCNT) on insulin secretion in the development of insulin resistance and to determine the underlying mechanism. PCNT expression was studied in different tissues of C57/B6 mice by reverse transcriptase-PCR and immunofluorescence. PCNT was highly expressed in organs involved in the regulation of metabolism, while cytoplasmic expression was only enriched in islet cells. PCNT expression was significantly lower in the central regions of insulin resistance (IR) mouse islets than in those of control mouse islets. PCNT expression was further studied in mouse MIN6 cells exposed to glucose stimulation, small interfering RNA (siRNA) against PCNT, and an ERK inhibitor (PD98095). The results revealed that PCNT expression in glucose-stimulated MIN6 cells reduced linearly with cytoplasmic insulin levels. MIN6 cells transfected with PCNT siRNA showed significantly decreased intracellular insulin and F-actin expression. The change in F-actin expression in MIN6 cells during PCNT siRNA interference showed a linear relationship with PCNT expression at different time points. The ERK inhibitor affected PCNT expression and F-actin expression linearly. The abnormal insulin secretion observed both in vivo and in vitro was associated with decreased PCNT expression, and F-actin was found to be the target of PCNT regulation.
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DOI:
10.1083/jcb.200908114
发表时间:
2010-01-25
期刊:
The Journal of cell biology
影响因子:
--
作者:
Delaval B;Doxsey SJ
通讯作者:
Doxsey SJ
DOI:
10.1016/s0140-6736(12)61729-2
发表时间:
2012-12-15
期刊:
Lancet (London, England)
影响因子:
--
作者:
Vos T;Flaxman AD;Naghavi M;Lozano R;Michaud C;Ezzati M;Shibuya K;Salomon JA;Abdalla S;Aboyans V;Abraham J;Ackerman I;Aggarwal R;Ahn SY;Ali MK;Alvarado M;Anderson HR;Anderson LM;Andrews KG;Atkinson C;Baddour LM;Bahalim AN;Barker-Collo S;Barrero LH;Bartels DH;Basáñez MG;Baxter A;Bell ML;Benjamin EJ;Bennett D;Bernabé E;Bhalla K;Bhandari B;Bikbov B;Bin Abdulhak A;Birbeck G;Black JA;Blencowe H;Blore JD;Blyth F;Bolliger I;Bonaventure A;Boufous S;Bourne R;Boussinesq M;Braithwaite T;Brayne C;Bridgett L;Brooker S;Brooks P;Brugha TS;Bryan-Hancock C;Bucello C;Buchbinder R;Buckle G;Budke CM;Burch M;Burney P;Burstein R;Calabria B;Campbell B;Canter CE;Carabin H;Carapetis J;Carmona L;Cella C;Charlson F;Chen H;Cheng AT;Chou D;Chugh SS;Coffeng LE;Colan SD;Colquhoun S;Colson KE;Condon J;Connor MD;Cooper LT;Corriere M;Cortinovis M;de Vaccaro KC;Couser W;Cowie BC;Criqui MH;Cross M;Dabhadkar KC;Dahiya M;Dahodwala N;Damsere-Derry J;Danaei G;Davis A;De Leo D;Degenhardt L;Dellavalle R;Delossantos A;Denenberg J;Derrett S;Des Jarlais DC;Dharmaratne SD;Dherani M;Diaz-Torne C;Dolk H;Dorsey ER;Driscoll T;Duber H;Ebel B;Edmond K;Elbaz A;Ali SE;Erskine H;Erwin PJ;Espindola P;Ewoigbokhan SE;Farzadfar F;Feigin V;Felson DT;Ferrari A;Ferri CP;Fèvre EM;Finucane MM;Flaxman S;Flood L;Foreman K;Forouzanfar MH;Fowkes FG;Franklin R;Fransen M;Freeman MK;Gabbe BJ;Gabriel SE;Gakidou E;Ganatra HA;Garcia B;Gaspari F;Gillum RF;Gmel G;Gosselin R;Grainger R;Groeger J;Guillemin F;Gunnell D;Gupta R;Haagsma J;Hagan H;Halasa YA;Hall W;Haring D;Haro JM;Harrison JE;Havmoeller R;Hay RJ;Higashi H;Hill C;Hoen B;Hoffman H;Hotez PJ;Hoy D;Huang JJ;Ibeanusi SE;Jacobsen KH;James SL;Jarvis D;Jasrasaria R;Jayaraman S;Johns N;Jonas JB;Karthikeyan G;Kassebaum N;Kawakami N;Keren A;Khoo JP;King CH;Knowlton LM;Kobusingye O;Koranteng A;Krishnamurthi R;Lalloo R;Laslett LL;Lathlean T;Leasher JL;Lee YY;Leigh J;Lim SS;Limb E;Lin JK;Lipnick M;Lipshultz SE;Liu W;Loane M;Ohno SL;Lyons R;Ma J;Mabweijano J;MacIntyre MF;Malekzadeh R;Mallinger L;Manivannan S;Marcenes W;March L;Margolis DJ;Marks GB;Marks R;Matsumori A;Matzopoulos R;Mayosi BM;McAnulty JH;McDermott MM;McGill N;McGrath J;Medina-Mora ME;Meltzer M;Mensah GA;Merriman TR;Meyer AC;Miglioli V;Miller M;Miller TR;Mitchell PB;Mocumbi AO;Moffitt TE;Mokdad AA;Monasta L;Montico M;Moradi-Lakeh M;Moran A;Morawska L;Mori R;Murdoch ME;Mwaniki MK;Naidoo K;Nair MN;Naldi L;Narayan KM;Nelson PK;Nelson RG;Nevitt MC;Newton CR;Nolte S;Norman P;Norman R;O'Donnell M;O'Hanlon S;Olives C;Omer SB;Ortblad K;Osborne R;Ozgediz D;Page A;Pahari B;Pandian JD;Rivero AP;Patten SB;Pearce N;Padilla RP;Perez-Ruiz F;Perico N;Pesudovs K;Phillips D;Phillips MR;Pierce K;Pion S;Polanczyk GV;Polinder S;Pope CA 3rd;Popova S;Porrini E;Pourmalek F;Prince M;Pullan RL;Ramaiah KD;Ranganathan D;Razavi H;Regan M;Rehm JT;Rein DB;Remuzzi G;Richardson K;Rivara FP;Roberts T;Robinson C;De Leòn FR;Ronfani L;Room R;Rosenfeld LC;Rushton L;Sacco RL;Saha S;Sampson U;Sanchez-Riera L;Sanman E;Schwebel DC;Scott JG;Segui-Gomez M;Shahraz S;Shepard DS;Shin H;Shivakoti R;Singh D;Singh GM;Singh JA;Singleton J;Sleet DA;Sliwa K;Smith E;Smith JL;Stapelberg NJ;Steer A;Steiner T;Stolk WA;Stovner LJ;Sudfeld C;Syed S;Tamburlini G;Tavakkoli M;Taylor HR;Taylor JA;Taylor WJ;Thomas B;Thomson WM;Thurston GD;Tleyjeh IM;Tonelli M;Towbin JA;Truelsen T;Tsilimbaris MK;Ubeda C;Undurraga EA;van der Werf MJ;van Os J;Vavilala MS;Venketasubramanian N;Wang M;Wang W;Watt K;Weatherall DJ;Weinstock MA;Weintraub R;Weisskopf MG;Weissman MM;White RA;Whiteford H;Wiersma ST;Wilkinson JD;Williams HC;Williams SR;Witt E;Wolfe F;Woolf AD;Wulf S;Yeh PH;Zaidi AK;Zheng ZJ;Zonies D;Lopez AD;Murray CJ;AlMazroa MA;Memish ZA
通讯作者:
Memish ZA
影响因子:
7.8
作者:
Martinez-Campos, Maruxa;Basto, Renata;Baker, James;Kernan, Maurice;Raff, Jordan W
通讯作者:
Raff, Jordan W
影响因子:
15.9
作者:
Ma, D;Shield, JPH;Kelsey, G
通讯作者:
Kelsey, G
影响因子:
3.7
作者:
Mühlhans J;Brandstätter JH;Giessl A
通讯作者:
Giessl A