Oligosaccharide model of the vascular endothelial glycocalyx in physiological flow.
Oligosaccharide model of the vascular endothelial glycocalyx in physiological flow.
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DOI：
10.1007/s10404-018-2037-5
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发表时间：
2018
影响因子：
2.8
通讯作者：
Ventikos Y
中科院分区：
文献类型：
--
作者：
Pikoula M;Tessier MB;Woods RJ;Ventikos Y
实验已经一致地揭示了内皮糖萼层在血管调节中的关键作用以及该层对机械转导途径的贡献。然而，糖萼介导流体剪切应力的确切机制仍然难以捉摸。本研究采用原子尺度的分子模拟的目的是调查的构象和取向特性的高度灵活的寡糖成分的糖萼和它们的适用性作为转导分子下的流体动力学负荷。流体流动被证明几乎没有影响的构象群体探索的寡糖，与静态（扩散）条件相比。然而，当与简单扩散相比时，聚糖表现出显著的取向变化，使其自身与流动方向对齐。它是聚糖（一种天冬酰胺氨基酸）的拴系末端，由于这种流动诱导的偏倚，其经历了构象变化。我们的研究结果表明，剪切流通过层可以有糖修饰的跨膜蛋白的构象特性的影响，从而作为一个mechanosensor。本文的在线版本（10.1007/s10404-018-2037-5）包含补充材料，可供授权用户使用。
Experiments have consistently revealed the pivotal role of the endothelial glycocalyx layer in vasoregulation and the layer’s contribution to mechanotransduction pathways. However, the exact mechanism by which the glycocalyx mediates fluid shear stress remains elusive. This study employs atomic-scale molecular simulations with the aim of investigating the conformational and orientation properties of highly flexible oligosaccharide components of the glycocalyx and their suitability as transduction molecules under hydrodynamic loading. Fluid flow was shown to have nearly no effect on the conformation populations explored by the oligosaccharide, in comparison with static (diffusion) conditions. However, the glycan exhibited a significant orientation change, when compared to simple diffusion, aligning itself with the flow direction. It is the tethered end of the glycan, an asparagine amino acid, which experienced conformational changes as a result of this flow-induced bias. Our results suggest that shear flow through the layer can have an impact on the conformational properties of saccharide-decorated transmembrane proteins, thus acting as a mechanosensor. The online version of this article (10.1007/s10404-018-2037-5) contains supplementary material, which is available to authorised users.
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影响因子：
2.8
作者：
Giantsos-Adams, Kristina M.;Koo, Andrew Jia-An;Song, Sukhyun;Sakai, Jiro;Sankaran, Jagadish;Shin, Jennifer H.;Garcia-Cardena, Guillermo;Dewey, C. Forbes, Jr.
通讯作者：
Dewey, C. Forbes, Jr.
影响因子：
8.6
作者：
KOPLIK, J;BANAVAR, JR;WILLEMSEN, JF
通讯作者：
WILLEMSEN, JF
影响因子：
4.4
作者：
JORGENSEN, WL;CHANDRASEKHAR, J;KLEIN, ML
通讯作者：
KLEIN, ML
影响因子：
4.4
作者：
ESSMANN, U;PERERA, L;PEDERSEN, LG
通讯作者：
PEDERSEN, LG
影响因子：
5.5
作者：
Koo, Andrew;Dewey, C. Forbes, Jr.;Garcia-Cardena, Guillermo
通讯作者：
Garcia-Cardena, Guillermo